Document 0473
 DOCN  M9650473
 TI    Monokine-mediated increase in human immunodeficiency virus type 1
       expression in chronically infected promonocyte- and T-cell-derived
       lines.
 DT    9605
 AU    Ho WZ; Tomassini N; Cherukuri R; Shun-D G; Song L; Lee HR; Douglas SD;
       Department of Pediatrics, Children's Hospital of Philadelphia,;
       University of Pennsylvania Medical School 19104, USA.
 SO    Clin Diagn Lab Immunol. 1994 Sep;1(5):517-25. Unique Identifier :
       AIDSLINE MED/96050865
 AB    The ACH-2 cell clone derived from a human T-cell line and chronically
       infected with human immunodeficiency virus 1 (HIV-1) and the U1 cell
       clone derived from a human promonocyte cell line and also chronically
       infected with HIV-1 produce HIV-1 in a response to stimulation with
       monokine-enriched supernatants prepared from highly purified populations
       of peripheral blood-derived human monocytes. Monokine-mediated
       expression of HIV-1 in these cell lines resulted in augmented virus
       production reflected by increases in reverse transcriptase (RT)
       activity, production of p24 antigen, and synthesis of major viral
       proteins. Examination of the cells by electron microscopy revealed
       numerous HIV-1 virions in the cells treated with the supernatants. This
       stimulation of virus production by monokine-enriched supernatants
       resulted in approximately 100-fold increases in RT activity and p24
       antigen expression in comparison with those in untreated U1 and ACH-2
       cells. Absorption of monokine-enriched supernatants with rabbit
       anti-tumor necrosis factor alpha antibody removed most, but not all, of
       the induced HIV-1 RT activity and p24 antigen expression in U1 and ACH-2
       cell lines, suggesting that tumor necrosis factor alpha in the
       monokine-enriched supernatants is a major factor in the induction of
       HIV-1 expression in these cells.
 DE    Adult  Cell Line/IMMUNOLOGY/ULTRASTRUCTURE/VIROLOGY  Human
       HIV-1/*IMMUNOLOGY  Interleukin-1/METABOLISM  Interleukin-6/METABOLISM
       Macrophages/IMMUNOLOGY/METABOLISM/VIROLOGY  Microscopy, Electron
       Monocytes/IMMUNOLOGY/METABOLISM/*VIROLOGY  Monokines/*PHARMACOLOGY
       Support, Non-U.S. Gov't  Support, U.S. Gov't, P.H.S.
       T-Lymphocytes/IMMUNOLOGY/METABOLISM/*VIROLOGY  Tumor Necrosis
       Factor/METABOLISM  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).