Document 0474
 DOCN  M9650474
 TI    Inhibitory effect of 3'-azido-3'-deoxythymidine on proliferative
       responsiveness of CD8+ lymphocytes to interleukin-2.
 DT    9605
 AU    Mercure L; Lalonde R; Phaneuf D; Brenner B; Wainberg MA; McGill
       University AIDS Centre, Montreal, Quebec, Canada.
 SO    Clin Diagn Lab Immunol. 1994 Jul;1(4):482-5. Unique Identifier :
       AIDSLINE MED/96050859
 AB    Although several studies have shown that 3'-azido-3'-deoxythymidine
       (AZT) is not toxic for CD4+ lymphocytes, its effect on CD8+ cells has
       never been studied in a systematic way. We purified CD8+ cells from the
       peripheral blood mononuclear cells of both human immunodeficiency virus
       (HIV)-seronegative and HIV-infected individuals by means of magnetic
       beads that had been coated with monoclonal antibodies. We report that
       AZT, but not two other nucleosides tested, inhibited the
       interleukin-2-dependent proliferation of CD8+ lymphocytes in a
       dose-dependent manner. No such effect was observed with regard to
       CD4(+)-enriched populations. The AZT-mediated antiproliferative effect
       did not appear to be related to either the CD4+ count or to prior
       treatment with this drug in the case of HIV-seropositive subjects.
 DE    Antiviral Agents/*IMMUNOLOGY/PHARMACOLOGY  Cell Division/IMMUNOLOGY
       CD8-Positive T-Lymphocytes/CYTOLOGY/DRUG EFFECTS/*IMMUNOLOGY
       Didanosine/IMMUNOLOGY/PHARMACOLOGY  Human  Interleukin-2/*ANTAGONISTS &
       INHIB/IMMUNOLOGY  Lymphocyte Transformation/DRUG EFFECTS  Support,
       Non-U.S. Gov't  Zalcitabine/ANALOGS &
       DERIVATIVES/IMMUNOLOGY/PHARMACOLOGY  Zidovudine/IMMUNOLOGY/*PHARMACOLOGY
       JOURNAL ARTICLE

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