Document 0476
 DOCN  M9650476
 TI    Markers of hepatitis C and B virus infections among blood donors in Ho
       Chi Minh City and Hanoi, Vietnam.
 DT    9605
 AU    Song P; Duc DD; Hien B; Nakata S; Chosa T; Watanabe J; Tsuda F; Murata
       K; Okamoto H; Institute for Clinical Research in Tropical Medicine, Bach
       Mai; Hospital, Hanoi, Vietnam.
 SO    Clin Diagn Lab Immunol. 1994 Jul;1(4):413-8. Unique Identifier :
       AIDSLINE MED/96050848
 AB    Blood donors in two cities in Vietnam were tested for markers of
       hepatitis C virus (HCV) and hepatitis B virus infections. Antibody to
       HCV was detected by passive hemagglutination with antigens of the second
       generation in 101 (20.6%) of 491 donors in Ho Chi Minh City; it was
       detected less frequently (P < 0.001) in donors in hanoi (4 [0.8%] of
       499). HCV RNA was tested for in donors with antibody by PCR with nested
       primers from the 5'-noncoding region and detected in 79 donors in Ho Chi
       Minh City and 4 donors in Hanoi. HCV RNA was genotyped by PCR with
       type-specific primers from the core gene. Of 83 HCV carriers from
       Vietnam, 24 (29%) were infected with HCV of genotype I/1a 19 (23%) were
       infected with II/1b, 4 (5%) were infected with III/2a, and 2 (2%) were
       infected with mixed genotypes (I/1a and II/1b); HCV genotypes in the
       remaining 34 (41%) donors, including all 4 donors in Hanoi, were not
       classifiable into I/1a, II/2a, IV/2b, or V/3a. Of the 10 isolates with
       unclassifiable genotypes, 2 showed substantial sequence divergence
       within the 5'-noncoding region from reported isolates with known
       genotypes (I/1a to 6a). An analysis of part of the core gene sequence
       indicated that six of the remaining isolates most likely represented new
       HCV genotypes. Hepatitis B surface antigen and the corresponding
       antibody, respectively, were detected in 15 (3.1%) and 234 (47.7%)
       donors in Ho Chi Minh City as well as 15 (3.0%) and 248 (49.7%) donors
       in Hanoi. These results indicate an extensive spread of HCV among Ho Chi
       Minh City donors and HCV of novel genotypes in vietnam.
 DE    Adult  Age Factors  Aged  Base Sequence  Biological Markers/BLOOD
       *Blood Donors  Female  Genotype  Hepatitis
       B/*BLOOD/EPIDEMIOLOGY/*IMMUNOLOGY  Hepatitis B Surface
       Antigens/IMMUNOLOGY  Hepatitis C/*BLOOD/EPIDEMIOLOGY/*IMMUNOLOGY
       Hepatitis C Antigens/IMMUNOLOGY  Hepatitis C Viruses/GENETICS  Human
       HIV/IMMUNOLOGY  HTLV-I/IMMUNOLOGY  Male  Middle Age  Molecular Sequence
       Data  Prevalence  Seroepidemiologic Methods  Support, Non-U.S. Gov't
       Vietnam/EPIDEMIOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).