Document 0484
 DOCN  M9650484
 TI    Changes in membrane enzymes and glycosphingolipids in lymphocytes from
       HIV-1--infected and noninfected intravenous drug users.
 DT    9605
 AU    Leoni LM; Losa GA; Laboratory of Cellular Pathology, Institute of
       Pathology,; Locarno, Switzerland.
 SO    J Acquir Immune Defic Syndr Hum Retrovirol. 1996 Feb 1;11(2):188-97.
       Unique Identifier : AIDSLINE MED/96147320
 AB    The amounts of cell-surface glycosphingolipids and plasma membrane
       enzymes produced on the peripheral blood mononuclear cells (PBMNCs)
       isolated from 101 intravenous drug users (IDUs), of whom 91 were HIV-1
       seropositive, were examined. Seronegative IDUs and age-matched healthy
       donors served as controls. The numbers of circulating CD3+, CD4+, and
       CD8+ T lymphocytes decreased during the advanced stages of the
       infection. There were also fewer CD4+ T-helper cells in
       HIV-1--seronegative IDU drug addicts. PBMNCs from HIV-1--seropositive
       subjects had abnormal surface enzyme kinetics. The phospholipase C had
       two pH optima, whereas the enzyme on normal cells has only one. The
       specific activity in cells from AIDS subjects was 4 times lower than
       that in normal PBMNCs. 5'-Nucleotidase showed a similar trend, whereas
       neutral endopeptidase activity did not correlate with the amounts of
       surface common acute lymphoblastic leukemia antigen (CALLA). These
       enzyme activities were decreased in HIV-seronegative IDUs. The
       subcellular distribution of enzymes and the profile of surface
       glycosphingolipids were also markedly changed, indicating the profound
       alterations in the membranes of PBMNCs from HIV-1--seropositive IDUs.
       These data suggest that intravenous drug use compromises the biochemical
       and structural integrity of the membrane surface of PBMNCs even before
       the onset of HIV.
 DE    Adolescence  Adult  Cell Membrane/ENZYMOLOGY  CD4 Lymphocyte Count
       CD4-Positive T-Lymphocytes/IMMUNOLOGY  CD8-Positive
       T-Lymphocytes/IMMUNOLOGY  Enzymes/*METABOLISM  Female
       Gangliosides/BLOOD  Glutathione Transferases/METABOLISM
       Glycosphingolipids/*BLOOD  Human  HIV
       Infections/*BLOOD/COMPLICATIONS/ENZYMOLOGY  HIV
       Seropositivity/BLOOD/ENZYMOLOGY/IMMUNOLOGY  *HIV-1  Immunophenotyping
       Lymphocytes/ENZYMOLOGY/*METABOLISM  Male  Membrane
       Metallo-Endopeptidase/METABOLISM  Middle Age  Peptide
       Peptidohydrolases/METABOLISM  Phospholipase C/METABOLISM  Sialic
       Acids/BLOOD  Substance Abuse,
       Intravenous/*BLOOD/COMPLICATIONS/ENZYMOLOGY  Support, Non-U.S. Gov't
       5'-Nucleotidase/METABOLISM  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).