Document 0492
 DOCN  M9650492
 TI    CD4+ T-lymphocyte lines developed from HIV-1-seropositive patients
       recognize different epitopes within the V3 loop.
 DT    9605
 AU    Ratto S; Sitz KV; Scherer AM; Loomis LD; Cox JH; Redfield RR; Birx DL;
       Henry M. Jackson Foundation, Rockville, MD 20850, USA.
 SO    J Acquir Immune Defic Syndr Hum Retrovirol. 1996 Feb 1;11(2):128-36.
       Unique Identifier : AIDSLINE MED/96147312
 AB    To define the epitopes present within the V3 loop sequence recognized by
       five HIV-1 envelope-specific T-cell lines, a panel of V3 LAI peptides
       bearing sequential truncations from both the N- and C-terminus was
       synthesized and tested for their ability to induce proliferation. Each
       individual T-cell line had a different pattern of response against the
       truncated V3 peptides, demonstrating the presence of a cluster of CD4+
       T-cell epitopes within the V3 loop. To assess the ability of these
       envelope-specific T-cell lines to recognize and proliferate in response
       to V3 loops of different viral strains, they were tested against a panel
       of heterologous V3 loop peptides derived from different viral genotypes
       within and outside of HIV-1 clade B. There was no proliferative response
       against heterologous V3 loops by any of the lines, demonstrating that
       recognition of the V3 epitopes is highly strain specific. One of the
       defined epitopes was shown to elicit a cytotoxic response as well,
       suggesting the multifaceted role that the CD4+ T cell might play in
       HIV-1 disease.
 DE    Amino Acid Sequence  Cell Line  Cells, Cultured  Cytotoxicity,
       Immunologic/IMMUNOLOGY  CD4-Positive T-Lymphocytes/*IMMUNOLOGY
       Epitopes/*IMMUNOLOGY  Gene Products, env/CHEMISTRY/IMMUNOLOGY
       Histocompatibility Antigens Class II/ANALYSIS  Histocompatibility
       Testing  Human  HIV Envelope Protein gp120/CHEMISTRY/*IMMUNOLOGY  HIV
       Infections/*IMMUNOLOGY  HIV Seropositivity/*IMMUNOLOGY
       HIV-1/*IMMUNOLOGY  Lymphocyte Transformation/IMMUNOLOGY  Molecular
       Sequence Data  Peptide Fragments/CHEMISTRY/*IMMUNOLOGY  Protein
       Precursors/CHEMISTRY/IMMUNOLOGY  T-Lymphocytes, Cytotoxic/IMMUNOLOGY
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).