Document 0494
 DOCN  M9650494
 TI    Selective effects of DNA damaging agents on HIV long terminal repeat
       activation and virus replication in vitro.
 DT    9605
 AU    Manome Y; Yao XJ; Kufe DW; Cohen EA; Fine HA; Division of Cancer
       Pharmacology, Dana Farber Cancer Institute,; Harvard Medical School,
       Boston, Massachusetts 02115, USA.
 SO    J Acquir Immune Defic Syndr Hum Retrovirol. 1996 Feb 1;11(2):109-16.
       Unique Identifier : AIDSLINE MED/96147310
 AB    Much attention has recently focused on the observation that UV light can
       activate the long terminal repeat (LTR) of the human immunodeficiency
       virus (HIV). Although the mechanism of LTR activation remains obscure,
       several lines of investigation have suggested that it is a result of
       activation of the NF-kappa B transcription factor(s) following signaling
       events related to generalized DNA damage. In this report, we present
       data demonstrating that HIV LTR activation is not a general consequence
       of cellular DNA damage, but rather a process unique to specific
       genotoxic stimuli, and that it does not necessarily depend on activation
       of NF-kappa B. Furthermore, we demonstrate that several of these agents
       can significantly increase HIV replication and accelerate CD4-positive
       lymphocyte cytotoxicity in vitro. These findings, therefore, could have
       clinical significance to AIDS patients with malignancies who are
       undergoing radiotherapy and chemotherapy.
 DE    Antineoplastic Agents/*PHARMACOLOGY  Comparative Study  CD4-Positive
       T-Lymphocytes/DRUG EFFECTS/RADIATION EFFECTS/  VIROLOGY  *DNA
       Damage/DRUG EFFECTS/RADIATION EFFECTS  DNA, Viral/*DRUG
       EFFECTS/RADIATION EFFECTS  Gene Expression Regulation, Viral/DRUG
       EFFECTS/RADIATION EFFECTS  Hela Cells/DRUG EFFECTS/RADIATION
       EFFECTS/VIROLOGY  Human  HIV Long Terminal Repeat/*DRUG
       EFFECTS/RADIATION EFFECTS  HIV-1/*PHYSIOLOGY  NF-kappa B/BIOSYNTHESIS
       Support, Non-U.S. Gov't  Support, U.S. Gov't, P.H.S.  Ultraviolet Rays
       *Virus Activation/DRUG EFFECTS/RADIATION EFFECTS  *Virus
       Replication/DRUG EFFECTS/RADIATION EFFECTS  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).