Document 0541
 DOCN  M9650541
 TI    Characterization of a transcriptional attenuator within the 5' R region
       of the human T cell leukemia virus type 1.
 DT    9605
 AU    Montagne J; Jalinot P; Laboratoire de Biologie Moleculaire et
       Cellulaire, CNRS UMR49,; Ecole Normale Superieure de Lyon, France.
 SO    AIDS Res Hum Retroviruses. 1995 Sep;11(9):1123-9. Unique Identifier :
       AIDSLINE MED/96089220
 AB    Several regulatory sequences have been characterized in the HTLV-I
       promoter. We report here identification of a sequence element downstream
       of the transcriptional start site within the first 52 nucleotides of the
       5' R region, which acts negatively on the activity of the HTLV-I
       promoter. Determination of the half-lives of the RNAs either including
       or lacking this sequence element showed that the observed effect
       intervenes at the transcriptional level. This negative element does not
       affect basal activity of the HTLV-I TATA box, but down-regulates
       transcription induced by strong activators. Thus, we propose that this
       so-called negative regulatory sequence functions as an attenuator of
       transcription.
 DE    Actins/GENETICS  Base Sequence  Down-Regulation (Physiology)  Genes,
       Regulator  Genes, Reporter  *Genes, Viral  Globin/GENETICS  Hela Cells
       Human  HTLV-I/*GENETICS  Molecular Sequence Data  Nucleic Acid
       Conformation  Promoter Regions (Genetics)  RNA, Viral/CHEMISTRY/GENETICS
       Support, Non-U.S. Gov't  Trans-Activation (Genetics)  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).