Document 0543
 DOCN  M9650543
 TI    Involvement of gag- and env-specific cytotoxic T lymphocytes in
       protective immunity to feline immunodeficiency virus.
 DT    9605
 AU    Flynn JN; Beatty JA; Cannon CA; Stephens EB; Hosie MJ; Neil JC; Jarrett
       O; Department of Veterinary Pathology, University of Glasgow,; Bearsden,
       Scotland.
 SO    AIDS Res Hum Retroviruses. 1995 Sep;11(9):1107-13. Unique Identifier :
       AIDSLINE MED/96089218
 AB    Definition of the immunological mechanisms involved in protective
       immunity against lentiviral infections is crucial to the development of
       an effective vaccine. The induction of gag- and env-specific
       cell-mediated immune responses was studied in cats following vaccination
       with whole inactivated feline immunodeficiency virus (FIV). Cats were
       immunized by inoculation with three doses of
       paraformaldehyde-inactivated FIV, derived from the feline lymphoid cell
       line, FL-4, which is persistently infected with the Petaluma isolate of
       FIV. Autologous or allogeneic skin fibroblasts either infected with
       recombinant FIV gag- or env-vaccinia virus or pulsed with FIV env
       peptides were used as targets in chromium-51 release assays. Effector
       cells were fresh peripheral blood mononuclear cells. Following the third
       immunization, all vaccinated cats, but none of the control cats
       immunized with adjuvant alone, had detectable FIV env-specific
       lymphocytotoxicity in their peripheral blood. Two cats also exhibited
       gag-specific activity. There was no recognition of either allogeneic
       skin fibroblasts infected with recombinant vaccinia virus or autologous
       target cells infected with wild-type vaccinia virus, indicating the
       specificity and MHC-restricted nature of the response. Vaccinated cats,
       but not control cats, were protected from challenge with the homologous
       Petaluma isolate of FIV. Partial epitope mapping of the env-specific
       cytotoxic response was performed using overlapping 10-amino acid
       peptides from the env V3 domain of FIV. This response appeared to be
       directed at env peptide 1 (RAISSWKQRN) and env peptide 3 (QRNRWEWRPD),
       which lie adjacent to a beta-turn within the V3 domain.(ABSTRACT
       TRUNCATED AT 250 WORDS)
 DE    Amino Acid Sequence  Animal  Antibodies, Viral/BLOOD  Cats  Epitope
       Mapping  Feline Acquired Immunodeficiency Syndrome/IMMUNOLOGY/PREVENTION
       &  CONTROL  Gene Products, env/GENETICS/*IMMUNOLOGY  Gene Products,
       gag/BLOOD/GENETICS/*IMMUNOLOGY  Immunodeficiency Virus,
       Feline/GENETICS/*IMMUNOLOGY  Molecular Sequence Data  Peptide
       Fragments/GENETICS/IMMUNOLOGY  Support, Non-U.S. Gov't  T-Lymphocytes,
       Cytotoxic/*IMMUNOLOGY  Vaccination  Vaccines, Inactivated/PHARMACOLOGY
       Viral Vaccines/PHARMACOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).