Document 0546
 DOCN  M9650546
 TI    Rapid molecular epidemiology of human immunodeficiency virus
       transmission.
 DT    9605
 AU    Delwart EL; Busch MP; Kalish ML; Mosley JW; Mullins JI; Aaron Diamond
       AIDS Research Center, New York University School of; Medicine, New York
       10016, USA.
 SO    AIDS Res Hum Retroviruses. 1995 Sep;11(9):1081-93. Unique Identifier :
       AIDSLINE MED/96089215
 AB    Close sequence homology between strains of HIV-1 have been used to
       corroborate cases of epidemiologically identified transmission. As an
       alternative to extensive DNA sequence analysis, genetic relateness
       between pairs of HIV quasispecies was estimated using the reduced
       electrophoretic mobilities of HIV-1 envelope DNA heteroduplexes through
       polyacrylamide gels. All six infections acquired in a dental practice in
       the late 1980s and four of six infections acquired through blood product
       transfusions and sexual contact in 1984-1985 could be rapidly
       identified. A rising level of genetic diversity within HIV-1 subtype B
       facilitated the detection of later transmission events. Transmission
       linkages could be detected up to 4 years following infection. The simple
       and rapid technique of DNA heteroduplex tracking can therefore assist
       epidemiological investigations of HIV transmission and potentially of
       other genetically variable infectious agents.
 DE    Base Sequence  Blood Donors  Blood Transfusion/ADVERSE EFFECTS  Dentists
       Disease Transmission, Professional-to-Patient  DNA Primers/GENETICS
       DNA, Viral/GENETICS  Epidemiology, Molecular  Genes, env  Human  HIV
       Infections/EPIDEMIOLOGY/*TRANSMISSION/VIROLOGY
       HIV-1/CLASSIFICATION/*GENETICS/ISOLATION & PURIF  Molecular Sequence
       Data  Nucleic Acid Heteroduplexes/GENETICS  Support, Non-U.S. Gov't
       Support, U.S. Gov't, P.H.S.  Time Factors  Variation (Genetics)  JOURNAL
       ARTICLE

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