Document 0553
 DOCN  M9650553
 TI    Enhanced susceptibility to human immunodeficiency virus infection in
       CD4+ T lymphocytes genetically deficient in CD43.
 DT    9605
 AU    Srinivas RV; Su T; Trimble LA; Lieberman J; Ardman B; Department of
       Infectious Diseases, St. Jude Children's Research; Hospital, Memphis,
       Tennessee 38105, USA.
 SO    AIDS Res Hum Retroviruses. 1995 Sep;11(9):1015-21. Unique Identifier :
       AIDSLINE MED/96089208
 AB    CD43 is a cell surface sialoglycoprotein expressed by most cells of
       hematopoietic origin, including all T lymphocytes. Elimination of CD43
       expression by gene targeting in the CEM T cell line results in its
       increased homotypic adhesion and binding to HIV-1 gp120. Here we report
       that the CD43-negative CEM cells show increased susceptibility to HIV-1
       infection and increased viral replication compared with the parental
       CD43+ CEM cell line. Increased HIV-1 replication also was observed in
       CEM cells with diminished CD43 expression secondary to functional
       inactivation of a single CD43 allele. The CD43- CEM cells were more
       susceptible to HIV-1-induced cytopathicity than their CD43+
       counterparts. HIV-1 replication also was increased in the CD43- CEM
       cells after transfection with the infectious HIV molecular clone pNL4-3.
       These data suggest that factors that diminish CD43 expression on T
       lymphocytes may enhance their susceptibility to HIV-1 infection.
 DE    Antigens, CD/GENETICS/*PHYSIOLOGY  Cell Line  Cell Survival
       Cytopathogenic Effect, Viral/IMMUNOLOGY  CD4-Positive
       T-Lymphocytes/*IMMUNOLOGY/*VIROLOGY  Gene Targeting  Human  HIV
       Infections/*ETIOLOGY/IMMUNOLOGY  HIV-1/PHYSIOLOGY/*PATHOGENICITY
       Kinetics  Sialoglycoproteins/*DEFICIENCY/GENETICS/IMMUNOLOGY  Support,
       Non-U.S. Gov't  Support, U.S. Gov't, P.H.S.  Virus Replication  JOURNAL
       ARTICLE

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