Document 0555
 DOCN  M9650555
 TI    Analysis and localization of cyclophilin A found in the virions of human
       immunodeficiency virus type 1 MN strain.
 DT    9605
 AU    Ott DE; Coren LV; Johnson DG; Sowder RC 2nd; Arthur LO; Henderson LE;
       AIDS Vaccine Program, National Cancer Institute, Frederick Cancer;
       Research and Development Center, Maryland 21702-1201, USA.
 SO    AIDS Res Hum Retroviruses. 1995 Sep;11(9):1003-6. Unique Identifier :
       AIDSLINE MED/96089206
 AB    Previous reports have shown that cyclophilin A (CyPA) is found to be
       specifically associated with human immunodeficiency virus type-1 (HIV-1)
       virions and is required for infectivity (Franke et al. Nature 372:359;
       Thali et al. Nature 372:363). We have examined CyPA associated with
       HIV-1MN virions. Virions from infected human lymphoid cells were
       analyzed by high-pressure liquid chromatography (HPLC), protein
       sequence, and immunoblot analysis. At least three forms of CyPA were
       found: an unmodified form, an N-terminally modified form, and an
       N-terminally modified form that migrates as a larger isoform on a
       reducing-SDS polyacrylamide gel. Using a protease digestion procedure,
       CyPA that is associated with virions was found to be located inside the
       viral membrane. Similar examination of SIVMne produced by HUT-78 human T
       cells did not detect specific incorporation of CyPA into SIV virions.
       Our results are consistent with the role of CyPA acting early in the
       infectious process of HIV-1.
 DE    Amino Acid Isomerases/*ANALYSIS/CHEMISTRY/METABOLISM  Amino
       Acids/ANALYSIS  Carrier Proteins/*ANALYSIS/CHEMISTRY/METABOLISM  Cell
       Line  Chromatography, High Pressure Liquid  Human
       HIV-1/*CHEMISTRY/GROWTH & DEVELOPMENT/METABOLISM  Immunoblotting
       Subtilisins  Support, U.S. Gov't, P.H.S.  Viral Envelope
       Proteins/ANALYSIS/METABOLISM  JOURNAL ARTICLE

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