Document 0569
 DOCN  M9650569
 TI    Retroviral vector-transduced cells expressing the core polyprotein
       induce feline immunodeficiency virus-specific cytotoxic T-lymphocytes
       from infected cats.
 DT    9605
 AU    Li J; Brown WC; Song W; Carpino MR; Wolf AM; Grant CK; Elder JH;
       Collisson EW; Department of Veterinary Pathobiology, Texas A&M
       University,; College Station 77843, USA.
 SO    Virus Res. 1995 Oct;38(2-3):93-109. Unique Identifier : AIDSLINE
       MED/96118168
 AB    The core polyprotein of feline immunodeficiency virus (FIV) was
       expressed in primary feline T-lymphocytes using a retroviral vector.
       These cells were used as antigen-presenting stimulator cells (APSC) for
       the in vitro induction of cytotoxic T-lymphocytes (CTL) from feline
       peripheral blood mononuclear cells (PBMC). CTL from 4 cats chronically
       infected with the Petaluma strain of FIV specifically lysed autologous
       FIV-infected targets in an MHC-restricted manner. The CD8 phenotype of
       more than 70% of the induced effector cells (97% for cells from one cat)
       was consistent with MHC class I-restricted cytotoxicity. In addition, it
       was possible to detect low levels of core polyprotein-specific lysis
       from effector cells of two of the FIV-infected cats. When observed, the
       level of lysis, measured as a percentage of specific 111In release, was
       lower for the transgenic gag-expressing targets than for FIV-infected
       targets. The difference in killing may reflect the low level of core CTL
       were not detected in either PBMC stimulated with cells transduced by a
       retroviral vector without the FIV gag sequence or PBMC from an
       uninfected cat stimulated with autologous transgenic APSC. The detection
       of FIV-specific CTL from infected cats following stimulation with
       transgenic APSC suggests a role for retroviral vectors in determining
       CTL specific for individual lentiviral proteins in protective immunity.
 DE    Animal  Base Sequence  Cats  Cell Line  DNA Primers  Feline Acquired
       Immunodeficiency Syndrome/*IMMUNOLOGY  Gene Products,
       gag/GENETICS/*IMMUNOLOGY  Gene Transfer  *Genes, Viral  Genetic Vectors
       Immunodeficiency Virus, Feline/GENETICS/*IMMUNOLOGY  Mice  Molecular
       Sequence Data  Phenotype  Recombinant Proteins/GENETICS/IMMUNOLOGY
       Retroviridae/GENETICS  Support, U.S. Gov't, P.H.S.  T-Lymphocytes,
       Cytotoxic/*IMMUNOLOGY  3T3 Cells  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).