Document 0572
 DOCN  M9650572
 TI    A new epitope presenting system displays a HIV-1 V3 loop sequence and
       induces neutralizing antibodies.
 DT    9605
 AU    Scodeller EA; Tisminetzky SG; Porro F; Schiappacassi M; De Rossi A;
       Chiecco-Bianchi L; Baralle FE; International Centre for Genetic
       Engineering and Biotechnology,; Trieste, Italy.
 SO    Vaccine. 1995 Sep;13(13):1233-9. Unique Identifier : AIDSLINE
       MED/96118133
 AB    The principal neutralizing domain, IGPGRAF sequence, from the V3-loop of
       HIV-1 was inserted in two positions on the surface of the protein that
       makes up the capside shell of the insect Flock House Virus. The hybrid
       proteins were expressed in insect cells via recombinant baculoviruses.
       Three different hybrids were used as immunogens: two with a single copy
       of the insert in different positions of the carrier protein and a third
       with two copies of the insert at the same positions as before. All
       hybrid proteins induced strong and broad specific immune response in
       guinea pigs against different V3-loop sequences. However, only one of
       the hybrid proteins was able to induce a strong neutralizing response
       against MN and IIIB HIV-1 isolates. Our results demonstrate that a very
       short peptide sequence of HIV-1 can constitute a valuable immunogen able
       to induce a neutralizing response if presented to the immune system in
       the context of the FHV capsomer structure.
 DE    Amino Acid Sequence  Animal  Antibodies, Viral/*BIOSYNTHESIS  Antibody
       Specificity  Antigen Presentation/*IMMUNOLOGY  Antigens,
       Viral/*IMMUNOLOGY  Baculoviridae/GENETICS  Base Sequence  Cell Line
       Epitopes/*IMMUNOLOGY  Genetic Vectors  Guinea Pigs
       HIV-1/GENETICS/*IMMUNOLOGY  Models, Molecular  Molecular Sequence Data
       Neutralization Tests  Spodoptera  Support, Non-U.S. Gov't  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).