Document 0573
 DOCN  M9650573
 TI    Antigenicity and immunogenicity of recombinant envelope glycoproteins of
       SIVmac32H with different in vivo passage histories.
 DT    9605
 AU    Hulskotte EG; Rimmelzwaan GF; Boes J; Bosch ML; Heeney JL; Norley SG; de
       Vries P; Osterhaus AD; Laboratory of Vaccine Development and Immune
       Mechanisms, National; Institute of Public Health and Environmental
       Protection,; Bilthoven, The Netherlands.
 SO    Vaccine. 1995 Sep;13(13):1187-97. Unique Identifier : AIDSLINE
       GENBANK/L35597
 AB    Shortly after infection of two rhesus monkeys (Macaca mulatta) either
       with a SIVmac32H challenge stock or with the same virus that had been
       passaged in another rhesus monkey for 11 months, SIV-envelope genes were
       cloned from their peripheral blood mononuclear cells and subsequently
       expressed by recombinant vaccinia viruses. The molecular weights and
       antigenicities of the thus produced envelope glycoproteins were largely
       identical to those of the native SIV. The envelope glycoprotein derived
       from the in vivo passaged virus proved to be poorly recognized by virus
       neutralizing monoclonal antibodies directed against one of the seven
       antigenic sites for which monoclonal antibodies were available.
       Immunization studies in rats showed that this protein was also less
       efficient in inducing antibodies against this antigenic site, and that
       it induced significantly lower levels of virus neutralizing antibodies
       than the other SIV-envelope glycoprotein. The immunogenicity of the
       SIV-envelope glycoprotein incorporated into immune stimulating complexes
       (iscoms) was compared to that of the same protein presented with Quil A
       or MDP-tsl.
 DE    Amino Acid Sequence  Animal  Antigens, Viral/*IMMUNOLOGY  Base Sequence
       Comparative Study  Female  Glycoproteins/*IMMUNOLOGY  Leukocytes,
       Mononuclear/VIROLOGY  Macaca mulatta  Molecular Sequence Data  Rats
       Recombinant Proteins/IMMUNOLOGY  Sequence Homology, Amino Acid  Serial
       Passage  Support, Non-U.S. Gov't  SAIDS Vaccines/*IMMUNOLOGY
       SIV/*IMMUNOLOGY  Vaccines, Synthetic/*IMMUNOLOGY  Vaccinia Virus  Viral
       Envelope Proteins/*IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).