Document 0578
 DOCN  M9650578
 TI    Experimental mucosal disease in cattle: changes of lymphocyte
       subpopulations in Peyer's patches and in lymphoid nodules of large
       intestine.
 DT    9605
 AU    Liebler EM; Kusters C; Pohlenz JF; Department of Veterinary Pathology,
       Veterinary School Hannover,; Germany.
 SO    Vet Immunol Immunopathol. 1995 Oct;48(3-4):233-48. Unique Identifier :
       AIDSLINE MED/96131983
 AB    Changes in the number and distribution of lymphocyte subtypes were
       investigated in Peyer's patches in the jejunum and ileum, and
       mucosa-associated lymphoid nodules in the proximal colon and rectum of
       cattle with end-stage mucosal disease. Mucosal disease had been induced
       experimentally in seven of 13 animals by inoculation with cytopathogenic
       bovine viral diarrhea virus (cp BVD-virus). For comparison, six
       clinically healthy, persistently viremic cattle were used. IgM+, IgA+,
       BoCD4+, BoCD8+ and gamma delta TCR+lymphocytes, and the cp BVD-viral
       antigen were visualized in tissue sections by immunohistochemistry. In
       cattle with mucosal disease, the size of lymphoid follicles was
       significantly decreased in all localizations resulting in decreased
       numbers of B-lymphocytes per average follicular area. In most animals
       domes were missing and epithelium was invaginated into the lymphoid
       follicles. Numbers of BoCD4+ and BoCD8 + T-lymphocytes were increased
       per mm2 of lymphoid follicle. Conversion of these counts into number of
       cells per average follicular area revealed, however, that the absolute
       number of BoCD4 + T-lymphocytes had decreased within lymphoid follicles
       and there was no distinct change of BoCD8 + T-lymphocytes in comparison
       to the controls. Interfollicular areas were less densely populated due
       to reduced numbers of BoCD4 + and BoCD8 + T-lymphocytes. cp BVD-viral
       antigen was detected predominantly in epithelial cells and in cells with
       dendritic morphology within lymphoid follicles. This may indicate that
       the severe depletion of B-lymphocytes in the lymphoid follicles is due
       to alterations of the microenvironment. The decrease of BoCD4 + and
       BoCD8 + T-lymphocytes does not support the hypothesis of T-cell-mediated
       tissue damage. Destruction of mucosa-associated lymphoid nodules does
       not only lead to local disruption of the gastrointestinal barrier, but
       will reduce the seeding of effector cells to the mucosa and therefore
       impair the defense mechanisms of the gastrointestinal barrier.
 DE    Animal  Antibodies, Monoclonal  Antibodies, Viral/ANALYSIS  Antigens,
       Viral/ANALYSIS  B-Lymphocytes/IMMUNOLOGY  Bovine Virus Diarrhea-Mucosal
       Disease/*IMMUNOLOGY/PATHOLOGY  Cattle  CD4-Positive
       T-Lymphocytes/IMMUNOLOGY  CD8-Positive T-Lymphocytes/IMMUNOLOGY
       Diarrhea Virus, Bovine Viral/*IMMUNOLOGY  Female  Immunoenzyme
       Techniques/VETERINARY  Intestinal Mucosa/IMMUNOLOGY  Intestine,
       Large/*IMMUNOLOGY  Intestine, Small/IMMUNOLOGY  Lymph Nodes/*IMMUNOLOGY
       Lymphocyte Count/VETERINARY  Lymphocyte Subsets/*IMMUNOLOGY  Peyer's
       Patches/*IMMUNOLOGY  Receptors, Antigen, T-Cell, gamma-delta/IMMUNOLOGY
       Support, Non-U.S. Gov't  Viremia/IMMUNOLOGY/VETERINARY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).