Document 0597
 DOCN  M9650597
 TI    Kruppel-associated box-mediated repression of RNA polymerase II
       promoters is influenced by the arrangement of basal promoter elements.
 DT    9605
 AU    Pengue G; Lania L; Department of Genetics, Molecular and General
       Biology, University; of Naples, Federico II, Italy.
 SO    Proc Natl Acad Sci U S A. 1996 Feb 6;93(3):1015-20. Unique Identifier :
       AIDSLINE MED/96165510
 AB    The evolutionarily conserved Kruppel-associated box (KRAB) is present in
       the N-terminal regions of more than one-third of all Kruppel-class zinc
       finger proteins. Recent experiments have demonstrated that the KRAB-A
       domain tethered to a promoter DNA by connecting to heterologous
       DNA-binding protein domain or targeted to a promoter-proximal RNA
       sequence acts as a transcriptional silencing of RNA polymerase II
       promoters. Here we show that expression of KRAB domain suppresses in
       vivo the activating function of various defined activating transcription
       factors, and we demonstrate that the KRAB domain specifically silences
       the activity of promoters whose initiation is dependent on the presence
       of a TATA box. Promoters whose accurate transcription initiation is
       directed by a pyrimidine-rich initiator element, however, are relatively
       unaffected. We also report in vitro transcription experiments indicating
       that the KRAB domain is able to repress both activated and basal
       promoter activity. Thus, the KRAB domain appears to repress the activity
       of certain promoters through direct communication with TATA
       box-dependent basal transcription machinery.
 DE    Base Sequence  Chloramphenicol Acetyltransferase/ANALYSIS/BIOSYNTHESIS
       Conserved Sequence  DNA-Binding Proteins/*METABOLISM  Enzyme Repression
       Hela Cells  Human  HIV Long Terminal Repeat  Molecular Sequence Data
       Oligodeoxyribonucleotides  Plasmids  *Promoter Regions (Genetics)
       Recombinant Proteins/BIOSYNTHESIS/METABOLISM  Repressor
       Proteins/METABOLISM  Restriction Mapping  RNA Polymerase
       II/*BIOSYNTHESIS/*GENETICS  Support, Non-U.S. Gov't  Transcription
       Factors/*METABOLISM  *Transcription, Genetic  Transfection  TATA Box
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
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