Document 0607
 DOCN  M9650607
 TI    Follicular dendritic cells productively infected with immunodeficiency
       viruses transmit infection to T cells.
 DT    9605
 AU    Sprenger R; Toellner KM; Schmetz C; Luke W; Stahl-Hennig C; Ernst M;
       Hunsmann G; Schmitz H; Flad HD; Gerdes J; et al; Department of
       Immunology and Cell Biology, Forschung Institut; Borstel, Germany.
 SO    Med Microbiol Immunol (Berl). 1995 Oct;184(3):129-34. Unique Identifier
       : AIDSLINE MED/96154932
 AB    Lymphoid organs have been proposed to function as the major reservoir
       for the human immunodeficiency virus type 1 (HIV-1). Within lymphatic
       tissues germinal centers represent foci of rapidly proliferating B cells
       governed by the interaction between B and T cells and follicular
       dendritic cells (FDC). Accumulating evidence suggests an important role
       of FDC in the pathophysiology of the acquired immunodeficiency syndrome.
       Direct proof for the infectibility of FDC with HIV-1 has been lacking
       until recently when we were able to demonstrate a CD4-independent
       infection of FDC in vitro. Here we report that in vitro HIV-1-infected
       human FDC do not only contain proviral DNA, but also produce the virus,
       and transmit the infection to T cells. Furthermore, electron
       microscopical studies on ex vivo isolated FDC from simian
       immunodeficiency virus (SIV)-infected rhesus monkeys revealed typical
       virus budding. In addition, FDC from SIV-infected rhesus monkeys
       transmitted the infection to T cells in vitro. Due to this central role
       within the immune response FDC may serve as preferential targets for HIV
       both by trapping of virions on their surfaces and by productive
       infection. During disease FDC become productively infected and may,
       thus, be regarded as crucial elements in viral dissemination.
 DE    Animal  Cells, Cultured  CD4-Positive T-Lymphocytes/*VIROLOGY  Dendritic
       Cells/ULTRASTRUCTURE/*VIROLOGY  Flow Cytometry  Human  HIV-1/*GROWTH &
       DEVELOPMENT  Lymph Nodes/CYTOLOGY  Macaca mulatta  Support, Non-U.S.
       Gov't  SIV/*GROWTH & DEVELOPMENT  Tonsil/CYTOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).