Document 0610
 DOCN  M9650610
 TI    Three antigenic regions in p17 of human immunodeficiency virus type 1
       (HIV-1) revealed by mouse monoclonal antibodies and human antibodies in
       HIV-1 carrier sera.
 DT    9605
 AU    Liu X; Ota A; Watanabe M; Ueda S; Saitoh A; Shinagawa H; Nakata A;
       Kurimura T; Wang X; Zhao Y; et al; Department of Immunochemistry,
       Research Institute for Microbial; Diseases, Osaka University, Japan.
 SO    Microbiol Immunol. 1995;39(10):775-85. Unique Identifier : AIDSLINE
       MED/96156439
 AB    We investigated the murine antibody response to recombinant p17 (rp17)
       of human immunodeficiency virus type 1 (HIV-1) and the human antibody
       response directed to p17 in HIV-1 infection. Three large peptides
       covering residues 12-29, 53-87 and 87-115 of p17 were synthesized. The
       cysteine residues 57 and 87 of peptide 53-87 were reoxidized to form a
       disulfide bridge. Eighteen out of 19 murine monoclonal anti-rp17
       antibodies had relatively high affinities (KA = 1.9 x 10(5)-1.4 x 10(8)
       M-1) with one of the 3 p17 peptides in the liquid phase. Each monoclonal
       antibody reacted only with one particular peptide and had no reactivity
       with the other 2 p17 peptides. All the monoclonal antibodies reacted
       with rp17 in the liquid phase with a reasonable degree of affinity (KA =
       2.0 x 10(5)-1.8 x 10(7) M-1). Four HIV-1 carrier sera, which were
       positive in ELISA using rp17 as the antigen, reacted positively in an
       ELISA using 3 p17 peptides which were used to titrate murine monoclonal
       antibodies. Murine monoclonal antibodies having specificity for the 3
       p17 peptides stained live HIV-1-infected cells by means of indirect
       membrane immunofluorescence, irrespective of their specificity. This
       suggests that the various portions of p17 (at least 3 regions of p17)
       were exposed on the surface of live infected cells, probably as short
       polypeptide chains.
 DE    Amino Acid Sequence  Animal  Antibodies, Monoclonal/BLOOD/*CHEMISTRY
       Binding Sites, Antibody  Carrier State/*IMMUNOLOGY/VIROLOGY
       Enzyme-Linked Immunosorbent Assay  *Epitope Mapping  Fluorescent
       Antibody Technique, Indirect  Gene Products, gag/CHEMISTRY/*IMMUNOLOGY
       Human  HIV Antibodies/*BLOOD  HIV Antigens/CHEMISTRY/*IMMUNOLOGY  HIV
       Infections/BLOOD/*IMMUNOLOGY  HIV-1/*IMMUNOLOGY  Mice  Molecular
       Sequence Data  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).