Document 0621
 DOCN  M9650621
 TI    Analysis of alloreactive helper T lymphocyte precursor frequencies.
       Influence of interleukin-2 produced by the stimulating cells.
 DT    9605
 AU    Reisaeter AV; Thorsby E; Brinchmann JE; Institute of Transplantation
       Immunology, National Hospital, Oslo,; Norway.
 SO    J Immunol Methods. 1996 Jan 16;189(1):65-72. Unique Identifier :
       AIDSLINE MED/96163545
 AB    In the determination of alloreactive helper T lymphocyte precursor
       frequencies (HTLpf) by limiting dilution analysis (LDA), peripheral
       blood mononuclear cells (PBMC) are used as stimulating cells.
       Interleukin-2 (IL-2) production by stimulating T cells constitute a
       source of error in these assays. We found that 100-150 Gy of gamma
       irradiation was required to abrogate IL-2 production by stimulating
       PBMC. This dose of irradiation, however, greatly reduced their
       allostimulatory capability. Here we describe how non-irradiated PBMC,
       immunomagnetically depleted of T cells and thus of IL-2 producing cells,
       can be used as stimulators in assays to determine alloresponsive HTLpf.
 DE    CD4-Positive T-Lymphocytes/IMMUNOLOGY  CD8-Positive
       T-Lymphocytes/IMMUNOLOGY  Dose-Response Relationship, Radiation  Gamma
       Rays  Human  Immunomagnetic Separation
       Interleukin-2/BIOSYNTHESIS/*PHYSIOLOGY/RADIATION EFFECTS  Lymphocyte
       Count/RADIATION EFFECTS  Lymphocyte Culture Test, Mixed  *Lymphocyte
       Transformation/RADIATION EFFECTS  Stem
       Cells/*IMMUNOLOGY/METABOLISM/RADIATION EFFECTS  T-Lymphocytes,
       Helper-Inducer/*IMMUNOLOGY/METABOLISM/RADIATION  EFFECTS  JOURNAL
       ARTICLE

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