Document 0634
 DOCN  M9650634
 TI    Evaluation of the reliability of 6 current anti-HIV-1/HIV-2 enzyme
       immunoassays.
 DT    9605
 AU    Weber B; Moshtaghi-Boronjeni M; Brunner M; Preiser W; Breiner M; Doerr
       HW; Institut fur Medizinische Virologie, Universitatskliniken,;
       Frankfurt/M., Germany.
 SO    J Virol Methods. 1995 Sep;55(1):97-104. Unique Identifier : AIDSLINE
       MED/96089773
 AB    The sensitivity for early detection of HIV antibodies and specificity of
       6 anti-HIV-1/HIV-2 screening enzyme immunoassays (ELISAs) currently on
       the market were investigated by testing a panel of 249
       well-characterized serum samples. The panel included sera from AIDS
       patients or children with congenital HIV infection, high-risk
       individuals and patients with conditions unrelated to AIDS. 'Tricky'
       sera (repeatedly positive results by ELISA and negative or indeterminate
       results by Western blot; n = 69) were also used in this evaluation along
       with 6 seroconversion panels. One second-generation assay (Biotest) and
       two third-generation assays (Abbott and Murex) showed the highest
       sensitivity for early detection of HIV-1 antibodies in seroconversion
       panels. A high specificity was achieved with the Cambridge Biotech
       (100%) and Ortho ELISA (99.4%). A relatively high rate of false-positive
       results was obtained with the Biotest (n = 10) and the Pasteur assays (n
       = 8) by testing 'tricky' sera and samples from high-risk individuals and
       from patients with other acute viral infections. In conclusion, it
       remains difficult to combine high specificity with an accurate detection
       of early seroconversion for anti-HIV-1/HIV-2 screening enzyme
       immunoassays.
 DE    Acquired Immunodeficiency Syndrome/IMMUNOLOGY/VIROLOGY  Adult  Child
       *Enzyme-Linked Immunosorbent Assay  Evaluation Studies  False Positive
       Reactions  Human  HIV Antibodies/*BLOOD/IMMUNOLOGY  HIV
       Seropositivity/IMMUNOLOGY/VIROLOGY  HIV-1/IMMUNOLOGY/*ISOLATION & PURIF
       HIV-2/IMMUNOLOGY/*ISOLATION & PURIF  IgM/BLOOD/IMMUNOLOGY  Reagent Kits,
       Diagnostic  Reproducibility of Results  Sensitivity and Specificity
       JOURNAL ARTICLE

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