Document 0635
 DOCN  M9650635
 TI    Colorimetric microtiter plate hybridization assay using monoclonal
       antibody for detection of an amplified human immunodeficiency virus
       target.
 DT    9605
 AU    Ferre-Aubineau V; Imbert-Marcille BM; Raffi F; Besse B; Loirat R;
       Billaudel S; Virology Laboratory, Nantes University Hospital, France.
 SO    J Virol Methods. 1995 Sep;55(1):145-51. Unique Identifier : AIDSLINE
       MED/96089778
 AB    Since detection of amplified polymerase chain reaction products is a
       complicated procedure, a colorimetric microtiter plate hybridization
       assay was developed to automate specific detection of amplified HIV
       targets. In this assay, hybridization is seen by an antibody reacting
       selectively with double-stranded DNA. One hundred and ten amplified
       products detected by a DNA enzyme immunoassay (DEIA) and by classical
       hybridization with a digoxigenin-labeling probe, detection sensitivities
       were less than 10 HIV targets per 10(6) cells. This study demonstrates
       the specificity and sensitivity of DEIA for detecting amplified HIV
       targets. The use of the same equipment as for ELISA and the complete
       automation of the procedure allow a large number of samples to be
       processed in the clinical laboratory.
 DE    Adult  Antibodies, Monoclonal/IMMUNOLOGY  Cell Line, Transformed
       Colorimetry  DNA, Viral/*ANALYSIS/*IMMUNOLOGY  Human  HIV
       Antibodies/*IMMUNOLOGY  HIV Seropositivity/DIAGNOSIS/IMMUNOLOGY
       HIV-1/GENETICS/*ISOLATION & PURIF  *Immunoenzyme Techniques  Polymerase
       Chain Reaction/*METHODS  JOURNAL ARTICLE

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