Document 0649
 DOCN  M9650649
 TI    Binding of distamycin and chromomycin to human immunodeficiency type 1
       virus DNA: a non-radioactive automated footprinting study.
 DT    9605
 AU    Feriotto G; Mischiati C; Bianchi N; Passadore M; Gambari R;
       Biotechnology Centre, Ferrara University, Italy.
 SO    Eur J Pharmacol. 1995 Jul 18;290(2):85-93. Unique Identifier : AIDSLINE
       MED/96171855
 AB    Sequence-selectivity of DNA-binding drugs was recently reported in a
       number of studies employing footprinting and gel retardation approaches.
       In this paper we studied sequence-selectivity of the binding of
       chromomycin and distamycin to DNA by performing DNase I footprinting and
       analysis of the cleaved fragments by the Pharmacia ALF DNA Sequencing
       System. As a model system we employed the long terminal repeat of the
       human immunodeficiency type 1 virus. The main conclusion of our
       experiments is that automated analysis of DNase I footprinting is a fast
       and reliable technique to study drugs-DNA interactions. The results
       obtained suggest that distamycin and chromomycin differentially interact
       with the long terminal repeat of the human immunodeficiency type 1
       virus; this differential binding depends upon the DNA sequences
       recognized. The data presented are consistent with a preferential
       binding of distamycin to DNA sequences of the binding sites of nuclear
       factor kappa B and transcription factor IID. By contrast, distamycin
       exhibits only weak binding to DNA sequences recognized by the
       promoter-specific transcription factor Sp1. Unlike distamycin,
       chromomycin preferentially interacts with the binding sites of the
       promoter-specific transcription factor Sp1.
 DE    Antibiotics, Antineoplastic/*METABOLISM  Antiviral Agents/*METABOLISM
       Automation  Base Sequence  Chromomycins/*METABOLISM
       Deoxyribonucleases/METABOLISM  Distamycins/*METABOLISM  *DNA
       Footprinting  DNA, Viral/*METABOLISM  Fluoresceins  Human
       HIV-1/*METABOLISM  Molecular Sequence Data  Polymerase Chain Reaction
       Support, Non-U.S. Gov't  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).