Document 0667
 DOCN  M9650667
 TI    Single-cell PCR analysis of TCR repertoires selected by antigen in vivo:
       a high magnitude CD8 response is comprised of very few clones.
 DT    9605
 AU    Maryanski JL; Jongeneel CV; Bucher P; Casanova JL; Walker PR; Ludwig
       Institute for Cancer Research, Swiss Institute for; Experimental Cancer
       Research, Epalinges, Switzerland.
 SO    Immunity. 1996 Jan;4(1):47-55. Unique Identifier : AIDSLINE MED/96158750
 AB    Taking advantage of a potent MHC class I-restricted response that allows
       the identification of antigen-selected CD8 T cells directly ex vivo, we
       characterized the antigen-specific T cell repertoires that develop in
       individual mice by single-cell PCR analysis. Each of the immune mice
       displayed distinct yet structurally similar TCR repertoires. The overall
       repertoire size was estimated to be in the range of 15-20 for most mice.
       No major differences were observed between primary and secondary
       responses. Moreover, for a hyperimmunized mouse the antigen-specific TCR
       repertoire expressed 8 months after the initial immunization was very
       similar to that found at the peak of the primary response. Our results
       demonstrate that a high magnitude immune response may be composed of
       very few clones, and that at least in the system analyzed, the memory
       response largely reflects the repertoire selected by the peak of the
       primary response.
 DE    Amino Acid Sequence  Animal  *Antigen Presentation  Base Sequence  Clone
       Cells/IMMUNOLOGY  CD8-Positive T-Lymphocytes/*IMMUNOLOGY  Immunity,
       Cellular  Mice  Mice, Inbred DBA  Molecular Sequence Data  Polymerase
       Chain Reaction  Receptors, Antigen, T-Cell/*IMMUNOLOGY  T-Lymphocyte
       Subsets/IMMUNOLOGY  JOURNAL ARTICLE

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