Document 0671
 DOCN  M9650671
 TI    Detection of human immunodeficiency virus type 1-specific memory
       cytotoxic T lymphocytes in freshly donated and frozen-thawed peripheral
       blood mononuclear cells.
 DT    9605
 AU    Huang XL; Fan Z; Liebmann J; Rinaldo C; Department of Infectious
       Diseases and Microbiology, University of; Pittsburgh Graduate School of
       Public Health, Pennsylvania 15261,; USA.
 SO    Clin Diagn Lab Immunol. 1995 Nov;2(6):678-84. Unique Identifier :
       AIDSLINE MED/96157368
 AB    Loss of anti-human immunodeficiency virus type 1 (HIV-1) memory
       cytotoxic T-lymphocyte (CTLm) responses is associated with disease
       progression in HIV-1 infection. In this study, nonspecific stimulation
       of peripheral blood mononuclear cells (PBMC) from HIV-1-infected
       homosexual men with anti-CD3 monoclonal antibody (MAb) was compared with
       antigen-specific stimulation with inactivated, autologous B
       lymphoblastoid cells (B-LCL) infected with a vaccinia virus vector
       encoding HIV-1 IIIb Gag, Pol, and Env (VV-GPE) for activation of
       HIV-1-specific CTLm responses in a bulk lysis assay and by precursor
       frequency analysis. The results show that VV-GPE-infected B-LCL
       stimulated on average 10-fold greater anti-HIV-1 CTLm activity, as
       detected in the bulk lysis assay, and 55-fold-greater CTLm precursor
       frequencies specific for the three HIV-1 structural proteins than did
       stimulation with anti-CD3 MAb. This effect was noted with both freshly
       donated and frozen-thawed PBMC. The lysis was mediated by CD8+ T cells
       and was restricted by the major histocompatibility class I complex.
       These data indicate that antigen-specific stimulation with
       VV-GPE-infected B-LCL is a highly efficient method for detection of
       anti-HIV-1 CTLm responses that is applicable to noncurrent prospective
       studies with frozen PBMC.
 DE    Acquired Immunodeficiency Syndrome/BLOOD/VIROLOGY  Antibodies,
       Monoclonal  Antibody Formation/IMMUNOLOGY  Antigens, Viral/IMMUNOLOGY
       B-Lymphocytes/IMMUNOLOGY/VIROLOGY  Blood Specimen Collection  Cell
       Survival/IMMUNOLOGY  Comparative Study  CD8-Positive
       T-Lymphocytes/IMMUNOLOGY  Freezing  Gene Products, env/IMMUNOLOGY  Gene
       Products, gag/IMMUNOLOGY  Gene Products, pol/IMMUNOLOGY
       Histocompatibility Antigens Class I/IMMUNOLOGY  Human  HIV-1/*ISOLATION
       & PURIF  Immunologic Memory  Immunophenotyping  Leukocytes,
       Mononuclear/*CYTOLOGY  Male  Support, Non-U.S. Gov't  Support, U.S.
       Gov't, P.H.S.  T-Lymphocytes, Cytotoxic/*VIROLOGY  Vaccinia
       Virus/IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
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