Document 0679
 DOCN  M9650679
 TI    Frequent presence of latent Epstein-Barr virus infection in peripheral T
       cell lymphomas. A review.
 DT    9605
 AU    Anagnostopoulos I; Hummel M; Stein H; Institute of Pathology, Klinikum
       Benjamin Franklin, Free; University Berlin, Germany.
 SO    Leuk Lymphoma. 1995 Sep;19(1-2):1-12. Unique Identifier : AIDSLINE
       MED/96116921
 AB    Two-hundred and four cases of peripheral T cell lymphoma (PTCL)
       occurring in Europeans without any sign of HIV-infection were
       investigated for their association with an Epstein-Barr virus (EBV)
       infection. Polymerase chain reaction (PCR) was applied for EBV-DNA
       detection, in situ hybridisation (ISH) for the cellular localization of
       EBV-encoded small nuclear RNA (EBER) and immediate early gene expression
       (BHLF) and immunohistology (IH) for the detection of EBV-encoded latent
       membrane protein 1 (LMP1) and EBV nuclear antigen 2 (EBNA2) expression.
       PCR and EBER-ISH produced congruent results in almost all cases with
       amplifiable DNA, leading to the finding of an overall frequency of EBV
       presence in 87/204 (42.6%) of the PTCL cases. Through EBER-ISH, the
       virus was identified to be exclusively present in small and blastic
       bystander lymphocytes in 29 cases, whilst an additional infection of
       neoplastic T cells was observed in the remaining 58 EBV-positive cases.
       The entity presenting with the most frequent EBV infection of tumour
       cells was that of angioimmunoblastic type PTCL, whilst the primary
       cutaneous PTCLs only seldom harbored the virus. Forty-eight of the
       EBV-positive TCLs showed an infection of a small proportion (1-20%) of
       the tumour cell population, whilst another ten cases, belonging to the
       pleomorphic TCL (PMTCL) group, displayed an infection of several to
       almost all neoplastic T cells (20-100%). Additional lytic EBV-infected
       cells could be detected in four cases by BHLF-ISH. LMP1 expression was
       present in a small proportion of the neoplastic T cells in 24 of the 58
       cases with tumour cell infection, whilst an EBNA2 expression was
       detectable only in one case. Some non-malignant EBV-infected
       B-immunoblasts and Hodgkin/Reed Sternberg-like cells also expressed LMP1
       in several cases. Our data imply a role of EBV in the pathogenesis of
       only a few PMTCL cases with predominant tumour cell infection, whilst
       the pathogenic significance of an EBV infection in the other PTCLs
       remains unclear due to the usually partial infection of the neoplastic
       cell component.
 DE    Antibodies, Monoclonal  Antibody Specificity  Antisense Elements
       (Genetics)  Genes, Immediate-Early  Genes, Viral  Herpesviridae
       Infections/EPIDEMIOLOGY/PATHOLOGY/*VIROLOGY  *Herpesvirus 4,
       Human/ISOLATION & PURIF  Human  Immunohistochemistry  In Situ
       Hybridization  Incidence  Lymphoma, T-Cell/PATHOLOGY/*VIROLOGY  Neoplasm
       Staging  Polymerase Chain Reaction  Retrospective Studies  RNA,
       Viral/ANALYSIS/GENETICS  Tumor Virus
       Infections/EPIDEMIOLOGY/PATHOLOGY/*VIROLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).