Document 0680
 DOCN  M9650680
 TI    Changes in viral expression and cytokine profile induced by a
       polyantigenic immunomodulator in HIV-infected peripheral blood
       mononuclear cells.
 DT    9605
 AU    Rios Z; Rios EO; Garcia MI; De Leon C; Guzman LM; Rodriguez W; Romero D;
       Hunter R; Department of Microbiology and Immunology, Universidad
       Central; del Caribe, School of Medicine, Bayamon, Puerto Rico
       00960-6032.
 SO    Cell Mol Biol (Noisy-le-grand). 1995;41 Suppl 1:S93-101. Unique
       Identifier : AIDSLINE MED/96171639
 AB    This is the first time, to our knowledge, that evidence is presented
       showing that a polyantigenic immunomodulator (PAI), acting as a
       biological response modifier, can either induce or suppress HIV
       expression depending on the viral load of infected PBMC. PAI consists of
       a mixture of inactivated bacteria with influenza virus vaccine. PBMC
       from HIV-infected patients (asymptomatic, age 22-36, symptomatic, age
       30-59 and pediatric, < 2 years old) were co-cultured with PHA-stimulated
       PBMC from uninfected individuals in medium containing IL-2 and PAI.
       Parallel co-cultures were carried out in a PAI-free medium. Cultures
       were fed with PHA-stimulated PBMC from uninfected donors on a weekly
       basis. HIV-p24 ag and cytokine profiles (IL-1 beta, IL-2, IL-4,
       IFN-gamma and TNF-alpha) were determined on supernatants on day 14.
       Peripheral blood samples from each patient were evaluated at the
       beginning of the experiment as to total CD3, total CD19, CD3/CD4,
       CD3/CD8, CD16/CD56, CD8/HLA-DR and CD8/CD38 markers through flow
       cytometry. PAI was able to induce viral expression (up to 11,881 pg/ml
       of p24 antigen) in cultures showing a low (less than 16 pg/ml) or no
       viral titer. In contrast, in those cultures with high viral titer
       (10(2)-10(5) pg/ml), a substantial reduction on the titer was observed
       upon exposure to PAI. PAI was able to induce the production of IFN-gamma
       and TNF-alpha while that of IL-4 and IL-1 beta was reduced. The
       predominant cell type detected in the blood samples of the studied
       subjects were CD8+, CD8+/CD38+ or CD8+/HLA-DR+.(ABSTRACT TRUNCATED AT
       250 WORDS)
 DE    Adjuvants, Immunologic/*PHARMACOLOGY  Adult  Age Factors  Antigens,
       Differentiation/ANALYSIS  Cells, Cultured  Comparative Study
       CD8-Positive T-Lymphocytes/DRUG EFFECTS/SECRETION/VIROLOGY  Female
       Human  HIV Infections/BLOOD/*IMMUNOLOGY  HIV-1/*PHYSIOLOGY  Infant
       Infant, Newborn  Influenza Vaccine/*PHARMACOLOGY  Interferon Type
       II/SECRETION  Interleukin-2/SECRETION  Interleukin-4/SECRETION
       Leukocytes, Mononuclear/*DRUG EFFECTS/SECRETION/VIROLOGY  Lymphocyte
       Count  Lymphocyte Subsets  Lymphokines/*SECRETION  Male  Middle Age
       Nucleosidases/ANALYSIS  Support, U.S. Gov't, P.H.S.  Tumor Necrosis
       Factor/SECRETION  Virus Replication/*DRUG EFFECTS  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).