Document 0682
 DOCN  M9650682
 TI    Distribution of linear antigenic epitopes on GP120 encoded in sibling
       clones of novel New York HIV-1 subtype B isolates.
 DT    9605
 AU    Riley JP; Pestano GA; Harewood K; Alfred LJ; Guyden J; Boto WM;
       Department of Biology and Chemistry, City College of the City;
       University of New York, NY 10031, USA.
 SO    Cell Mol Biol (Noisy-le-grand). 1995;41 Suppl 1:S83-91. Unique
       Identifier : AIDSLINE MED/96171638
 AB    We have initiated studies to characterize the predominant subtypes of
       HIV-1 which account for infections in a defined cohort of intravenous
       (IV) drug addicts. A region of ENV encoding the C2 to the V5 regions was
       amplified from the leukocytes of two subjects currently enrolled in a
       methadone maintenance program at the Addiction Research and Treatment
       Corporation (ARTC), in Brooklyn, New York. This region of the viral
       genome encodes the principal neutralizing determinant (PND) located in
       the V3 loop, the immunogenic CD4-binding site, and six other linear
       antigenic epitopes in the envelope glycoprotein, gp120. Phylogenetic
       tree analysis of the nucleotide sequences showed that the sibling clones
       RT1.4, RT1.15, RT1.17, RT1.21 and RT3.6, RT3.10, RT3.11, RT3.12 and
       RT3.15 derived from the isolates, RT1 and RT3, respectively, cluster
       with group B viruses at 99% confidence level. Marked intra-patient and
       inter-patient sequence variation was apparent in the V3 loop. The
       divergence included the presence of a previously unreported hexapeptide
       GPWGTF at the cap of the loop in the clones from RT1. The North American
       consensus hexapeptide, GPGRAF, was identified in the cap of the loop
       from the clones of RT3. Four of the five sibling clones from RT3 were
       closely related whereas the other clone, RT3.15, displayed five amino
       acid mutations downstream of the V3 cap. To assess the effect of
       sequence variation on the distribution of linear antigenic epitopes,
       complementary computer software programs, were used to analyze the gp120
       residues. Eight analogous antigenic epitopes were identified in the
       clones from both isolates despite the marked divergence in the primary
       sequences.(ABSTRACT TRUNCATED AT 250 WORDS)
 DE    Amino Acid Sequence  Antigenic Variation/*GENETICS  Cohort Studies
       Comorbidity  Comparative Study  Enzyme-Linked Immunosorbent Assay
       Epitopes/*IMMUNOLOGY  Human  HIV Antibodies/BLOOD/IMMUNOLOGY  HIV
       Envelope Protein gp120/CHEMISTRY/GENETICS/*IMMUNOLOGY  HIV
       Infections/EPIDEMIOLOGY/TRANSMISSION/*VIROLOGY
       HIV-1/CLASSIFICATION/GENETICS/*IMMUNOLOGY/ISOLATION & PURIF  Molecular
       Sequence Data  New York City/EPIDEMIOLOGY  Peptide
       Fragments/GENETICS/IMMUNOLOGY  Phylogeny  Polymerase Chain Reaction
       Protein Structure, Tertiary  Risk Factors  Sequence Alignment  Sequence
       Homology, Amino Acid  Substance Abuse Treatment Centers/STATISTICS &
       NUMER DATA  Substance Abuse, Intravenous/EPIDEMIOLOGY  Support, Non-U.S.
       Gov't  Support, U.S. Gov't, Non-P.H.S.  Support, U.S. Gov't, P.H.S.
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).