Document 0686
 DOCN  M9650686
 TI    N-acetylcysteine (NAC) enhances interleukin-2 but suppresses
       interleukin-4 secretion from normal and HIV+ CD4+ T-cells.
 DT    9605
 AU    Eylar EH; Baez I; Vazquez A; Yamamura Y; Department of Biochemistry and
       Microbiology, Ponce School of; Medicine, Puerto Rico 00732.
 SO    Cell Mol Biol (Noisy-le-grand). 1995;41 Suppl 1:S35-40. Unique
       Identifier : AIDSLINE MED/96171633
 AB    We find that purified CD4+ T cells from 30 HIV+ individuals have a
       suppressed Interleukin-4 (IL-4) production compared to normal controls
       regardless of activator (anti-CD3 or Con A) or co-activator [phorbol
       ester (PMA or anti-CD28)], generally by 2-4 fold. In every case, the
       cells producing IL-4 respond more strongly to anti-CD28 co-activation
       than to PMA, ie, 1150 pg/ml compared to 2070 pg/ml for controls and 398
       pg/ml compared to 1250 pg/ml for HIV+ cells, respectively. In contrast,
       anti-CD3 with PMA gives a more vigorous IL-2 response than with
       anti-CD28, ie, 37.3 ng/ml compared to 12.3 ng/ml for controls and 28.5
       ng/ml versus 15.1 ng/ml for HIV+ cells, respectively. These data are not
       compatible with the TH1/TH2 switch hypothesis since IL-4 production is
       decreased, not increased for CD4+ HIV+ T-cells and while IL-2 production
       is decreased with PMA, it is not decreased significantly with anti-CD28.
       Interestingly, 5 mM N-acetylcysteine (NAC) acts as an immunoenhancer;
       mitogenesis was enhanced 2 fold or more in general for control and HIV+
       CD4+ T-cells and IL-2 production was enhanced 2-3 fold for anti-CD3
       (with PMA or anti-CD28) for both controls and HIV+ CD4+ cells. However,
       NAC suppressed IL-4 production induced by anti-CD3 and anti-CD28 in both
       control and HIV+ CD4+ T cells. In the other cases, it produced in
       general no significant change.(ABSTRACT TRUNCATED AT 250 WORDS)
 DE    Acetylcysteine/*PHARMACOLOGY  Adult  Antibodies, Monoclonal/PHARMACOLOGY
       Antigens, CD28/PHYSIOLOGY  Comparative Study  Concanavalin
       A/PHARMACOLOGY  Hispanic Americans  Human  HIV Infections/*IMMUNOLOGY
       Interleukin-2/*SECRETION  Interleukin-4/*SECRETION  Lymphocyte
       Transformation  Muromonab-CD3/PHARMACOLOGY  Support, U.S. Gov't, P.H.S.
       Tetradecanoylphorbol Acetate/PHARMACOLOGY  Th1 Cells/*DRUG
       EFFECTS/SECRETION  Th2 Cells/*DRUG EFFECTS/SECRETION  JOURNAL ARTICLE

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