Document 0687
 DOCN  M9650687
 TI    Suppressed proliferative response and interleukin-2 production in
       hispanic HIV+ and AIDS T-cell subsets.
 DT    9605
 AU    Eylar EH; Baez I; Vazquez A; Colon-Martinez SL; Yamamura Y; Department
       of Biochemistry, Ponce School of Medicine 00732,; Puerto Rico.
 SO    Cell Mol Biol (Noisy-le-grand). 1995;41 Suppl 1:S25-33. Unique
       Identifier : AIDSLINE MED/96171632
 AB    We find that interleukin-2 (IL-2) production is severely depressed
       (80-90%) in AIDS T-cells (CD4+ or CD8+) stimulated with anti-CD3 or Con
       A together with phorbol ester (PMA) or anti-CD28 coactivation. Likewise,
       the proliferative response of CD4+ T-cells was suppressed, from a mean
       of 24.6% (HIV+) to 59.1% (AIDS) for PMA with activators OKT3 (anti-CD3),
       Con A, enterotoxin B or pokeweed mitogen, and 20.2% (HIV+) to 77.8%
       (AIDS) with anti-CD28 co-activation. Similar degrees of suppression were
       found with the CD8+ T-cells except for a much greater suppression at the
       HIV+ stage with anti-CD28 (57.7%), approximately 2.5 times higher than
       for PMA coactivation. However, when proliferation was induced by the two
       coactivators combined (PMA plus anti-CD28), much less suppression was
       observed: 8.5% (HIV+) to 19.0% (AIDS) for CD4+ cells and 8.2% to 26.5%,
       respectively, for CD8+ cells. The data suggest that during HIV infection
       the CD28 pathway becomes most defective, but can be bypassed to some
       extent by the less-impaired PMA pathway. The IL-2 (+PMA) signal in HIV+
       and AIDS cells was also significantly less suppressed suggesting that
       the disregulation in HIV infection is more prominent prior to the IL-2
       stage of the mitogenic pathway. It is remarkable that the CD4+ and CD8+
       T-cells at both the HIV+ and AIDS stages generally show the same degree
       of suppression with all the various activators and coactivators
       used.(ABSTRACT TRUNCATED AT 250 WORDS)
 DE    Acquired Immunodeficiency Syndrome/IMMUNOLOGY  Adult  Antigens,
       CD28/PHYSIOLOGY  Comparative Study  Concanavalin A/PHARMACOLOGY  CD4
       Lymphocyte Count  CD4-Positive T-Lymphocytes/IMMUNOLOGY  CD8-Positive
       T-Lymphocytes/IMMUNOLOGY  Enterotoxins/PHARMACOLOGY  *Hispanic Americans
       Human  HIV Infections/*IMMUNOLOGY  Interleukin-2/*BIOSYNTHESIS
       *Lymphocyte Transformation  Pokeweed Mitogens/PHARMACOLOGY  Severity of
       Illness Index  Support, U.S. Gov't, P.H.S.  T-Lymphocyte
       Subsets/*IMMUNOLOGY  Tetradecanoylphorbol Acetate/PHARMACOLOGY  JOURNAL
       ARTICLE

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