Document 0691
 DOCN  M9650691
 TI    Anti-CD4 cytotoxic T lymphocyte (CTL) activity in HIV+ patients: flow
       cytometric analysis.
 DT    9605
 AU    Yamamura Y; Rodriguez N; Schwartz A; Eylar E; Yano N; Ponce School of
       Medicine, AIDS Research Program, Puerto Rico; 00732.
 SO    Cell Mol Biol (Noisy-le-grand). 1995;41 Suppl 1:S133-44. Unique
       Identifier : AIDSLINE MED/96171643
 AB    A new cell proliferation analysis by flow cytometry was applied to the
       mitogen induced cultures of peripheral blood mononuclear cells (PBMC)
       from either normal, healthy donors or those individuals who are infected
       by human immunodeficiency virus, type 1 (HIV-1). While
       phytohemagglutinin (PHA) stimulation of normal PBMC (nPBMC) yielded
       propagation of both CD4 (nCD4) and CD8 (nCD8) T cell subsets, similar
       activation of PBMC from certain HIV-1-infected individuals (HIV-PBMC)
       produced active proliferation of CD8 (HIV-CD8) cells but varying degrees
       of CD4 (HIV-CD4) cell destruction. However, no measurable viral p24
       antigen was produced extracellularly. On the other hand, when the
       purified HIV-CD4 cells were similarly activated, no such cell death was
       noted and high titer p24 was detected in the culture supernatants.
       Addition of exogenous IL-2 to either HIV-PBMC or HIV-CD4 cultures, did
       not alter either CD4 cell death or HIV-1 p24 production. Isolated
       HIV-CD8 killed not only HIV-CD4 but also nCD4, when co-cultured and less
       proliferative fractions of CD4 cell population was the more preferred
       targets of the HIV-CD8 CTL activity. The presence of anti-CD4 CTL
       activity was closely associated with high CD8, but not with CD4 counts,
       of the HIV+ patients.
 DE    Cell Division  Cell Separation  Cells, Cultured  Comparative Study
       Cytotoxicity, Immunologic  CD4 Lymphocyte Count  CD4-Positive
       T-Lymphocytes/*IMMUNOLOGY/VIROLOGY  *Flow Cytometry  Human  HIV Core
       Protein p24/BIOSYNTHESIS  HIV Infections/BLOOD/*IMMUNOLOGY
       HIV-1/*PHYSIOLOGY  Lymphocyte Count  *Lymphocyte Transformation/DRUG
       EFFECTS  Phytohemagglutinins/PHARMACOLOGY  Support, U.S. Gov't, P.H.S.
       T-Lymphocytes, Cytotoxic/DRUG EFFECTS/*IMMUNOLOGY/VIROLOGY  Virus
       Replication  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).