Document 0700
 DOCN  M9650700
 TI    A simple quantitative culture of Malassezia spp. in HIV-positive
       persons.
 DT    9605
 AU    Pechere M; Remondat C; Bertrand C; Didierjean L; Saurat JH; Department
       of Dermatology, Geneva University Hospital,; Switzerland.
 SO    Dermatology. 1995;191(4):348-9. Unique Identifier : AIDSLINE
       MED/96159488
 AB    BACKGROUND: Etiological role of Malassezia spp. remains controversial in
       certain skin diseases. OBJECTIVE: To adapt a 'tape method' for
       quantitative culture of Malassezia spp. METHOD: Samples for culture were
       taken from clinically normal forehead skin of HIV-positive and negative
       persons by stripping with a tape that was then placed on Leeming &
       Notman medium. The number of colonies was counted after 14 days.
       RESULTS: 74/78 (94.8%) cultures were positive, for a median count of 9
       CFU/tape (range 0 to > 200). High skin density of Malassezia spp.,
       defined as more than 100 CFU/tape, was found in 7/38 (18.4%)
       HIV-positive persons and was absent (0/40) in the HIV-negative group (p
       < 0.01). CONCLUSION: The method used is simple, unexpensive and
       reliable. High Malassezia spp. density was only found in HIV-positive
       patients.
 DE    Colony Count, Microbial  Culture Media  Forehead  Human  HIV
       Seronegativity  HIV Seropositivity/*MICROBIOLOGY  Malassezia/*ISOLATION
       & PURIF  Mycology/INSTRUMENTATION/METHODS  Reproducibility of Results
       Skin/*MICROBIOLOGY  JOURNAL ARTICLE

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