Document 0701
 DOCN  M9650701
 TI    HIV-regulated diphtheria toxin A chain gene confers long-term protection
       against HIV type 1 infection in the human promonocytic cell line U937.
 DT    9605
 AU    Dinges MM; Cook DR; King J; Curiel TJ; Zhang XQ; Harrison GS; Department
       of Pediatrics, University of Minnesota Hospital and; Clinic, Minneapolis
       55455, USA.
 SO    Hum Gene Ther. 1995 Nov;6(11):1437-45. Unique Identifier : AIDSLINE
       MED/96139257
 AB    Gene therapy approaches have recently been investigated for the
       treatment of acquired immunodeficiency syndrome (AIDS), both in
       preclinical and clinical studies, because more traditional antiviral
       agents have proven to be of limited effectiveness. We have previously
       shown that long-term protection against both laboratory and clinical
       isolates of human immunodeficiency virus type 1 (HIV-1) was conferred by
       HIV-regulated diphtheria toxin A (DT-A) chain in a human T cell line.
       Because the monocyte/macrophage cell is an important reservoir for HIV-1
       in infected individuals, we sought here to determine whether
       HIV-regulated DT-A would also be effective in the promonocytic cell line
       U937. We report here that long-term protection, conferred by
       HIV-regulated DT-A, was observed in U937 cells, but that protection was
       dependent on the stock of HIV IIIB used for challenge. HIV production
       was measured by p24 assays, polymerase chain reaction (PCR) for HIV vif,
       gag, and reverse transcriptase (RT) sequences, and cocultivation with
       peripheral blood mononuclear cells (PBMCs). Complete protection was seen
       in DT-A-transduced cells with a stock of IIIB propagated on H9 cells and
       titered on peripheral blood mononuclear cells (PBMCs), while protection
       in these same cells with a second stock of IIIB, propagated and titered
       on H9 cells, was only partial and dose dependent.
 DE    Antigens, CD4/IMMUNOLOGY  Cell Line  Cell Survival  Diphtheria
       Toxin/*GENETICS/IMMUNOLOGY  DNA, Viral/ANALYSIS  Flow Cytometry  *Gene
       Expression Regulation, Viral  *Gene Transfer  Genetic Vectors  Human
       HIV-1/*GENETICS/PHYSIOLOGY  Monocytes/VIROLOGY  Peptide
       Fragments/*GENETICS/IMMUNOLOGY  Polymerase Chain Reaction  Support,
       Non-U.S. Gov't  Support, U.S. Gov't, P.H.S.  Trans-Activation (Genetics)
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).