Document 0704
 DOCN  M9650704
 TI    Karyotyping of fluconazole-resistant yeasts with phenotype reported as
       Candida krusei or Candida inconspicua.
 DT    9605
 AU    Essayag SM; Baily GG; Denning DW; Burnie JP; Department of Medical
       Microbiology, Manchester Royal Infirmary,; University of Manchester,
       United Kingdom.
 SO    Int J Syst Bacteriol. 1996 Jan;46(1):35-40. Unique Identifier : AIDSLINE
       MED/96138963
 AB    The yeasts Candida krusei and Candida inconspicua have similar
       phenotypes, which may make discrimination of these organisms difficult.
       In this study we determined the karyotypes of 51 isolates of these two
       yeast species by contour-clamped homogeneous electric field
       electrophoresis. We found that the 43 isolates that had the C. krusei
       phenotype had three karyotype-specific characteristics. These isolates
       produced either two or three bands between 2,000 and 3,000 kb and no
       band between 1,300 and 2,000 kb, and there was either a single bright
       band at 1,300 or 1,200 kb or two separate bands at 1,300, 1,200, or
       1,100 kb. Using this technique, we were able to distinguish 27 different
       C. krusei types on the basis of band variations. The seven isolates
       identified as C. inconspicua on the basis of phenotype differed in that
       they produced at least one band between 1,300 and 2,000 kb. These
       isolates produced six to nine bands, in contrast to C. krusei strains,
       which produced three to six bands. The MIC of fluconazole for all of the
       isolates was at least 12.5 mg/liter, as determined by a broth dilution
       method.
 DE    Antifungal Agents/*PHARMACOLOGY  Candida/CLASSIFICATION/DRUG
       EFFECTS/*GENETICS  Candidiasis/COMPLICATIONS/*MICROBIOLOGY  Chromosomes,
       Fungal  Drug Resistance, Microbial  DNA, Fungal/ANALYSIS
       Electrophoresis, Agar Gel  Fluconazole/*PHARMACOLOGY  Human  HIV
       Seropositivity/COMPLICATIONS/DRUG THERAPY/MICROBIOLOGY  Karyotyping
       Phenotype  JOURNAL ARTICLE

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