Document 0710
 DOCN  M9650710
 TI    Highly attenuated SIVmac142 is immunogenic but does not protect against
       SIVmac251 challenge.
 DT    9605
 AU    Denesvre C; Le Grand R; Boissin-Cans F; Chakrabarti L; Hurtrel B; Vaslin
       B; Dormont D; Sonigo P; ICGM-CNRS UPR415, Institut Cochin de Genetique
       Moleculaire,; Paris, France.
 SO    AIDS Res Hum Retroviruses. 1995 Nov;11(11):1397-406. Unique Identifier :
       AIDSLINE MED/96159138
 AB    We report here the use of the highly attenuated SIVmac142 clone, unable
       to establish permanent infection of rhesus macaques, in a vaccine trial.
       Four rhesus macaques were immunized over a long time period with HUT-78
       cells infected with wild-type SIVmac142 or, in order to reinforce the
       safety use of the vaccine, a deleted mutant with similar in vitro
       infectivity. The first two injections were done with living cells and
       the remaining boosts with cells emulsified in muramyl dipeptide
       adjuvant. Three control macaques were injected with uninfected HUT-78
       cells. Over 3 years, we have been unable except once to detect viral
       infections by three methods. However, antibodies directed against the
       viral Gag proteins and envelope glycoproteins were detected by
       immunoblots and/or in vitro neutralization assays. All macaques were
       challenged intravenously with a low dose (10 animal infectious doses) of
       a highly pathogenic biological clone of SIVmac251 grown on macaque
       PBMCs. All seven animals became persistently viremic following
       challenge. The cell-associated viral loads of the vaccinated monkeys
       were not reduced relative to those of unvaccinated controls during the
       first weeks postchallenge even if vaccinated monkeys did not present a
       transient CD4 decrease. Thus, our data reinforced the notion that the
       efficacy of live attenuated SIV requires the establishment of persistent
       infection.
 DE    Animal  Antibodies, Viral/BIOSYNTHESIS/IMMUNOLOGY  Base Sequence  Cell
       Line  DNA Primers  Female  Human  Macaca mulatta  Male  Molecular
       Sequence Data  Mutation  Simian Acquired Immunodeficiency
       Syndrome/*PREVENTION & CONTROL  Support, Non-U.S. Gov't  SAIDS
       Vaccines/*IMMUNOLOGY  SIV/*IMMUNOLOGY/ISOLATION & PURIF  Vaccines,
       Attenuated/IMMUNOLOGY  JOURNAL ARTICLE

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