Document 0713
 DOCN  M9650713
 TI    Structural modification of serum vitamin D3-binding protein and
       immunosuppression in AIDS patients.
 DT    9605
 AU    Yamamoto N; Naraparaju VR; Srinivasula SM; Laboratory of Cancer
       Immunology and Molecular Biology, Albert; Einstein Cancer Center,
       Philadelphia, Pennsylvania 19141, USA.
 SO    AIDS Res Hum Retroviruses. 1995 Nov;11(11):1373-8. Unique Identifier :
       AIDSLINE MED/96159135
 AB    A serum glycoprotein, vitamin D3-binding protein (Gc protein), can be
       converted by beta-galactosidase of stimulated B lymphocytes and
       sialidase of T lymphocytes to a potent macrophage-activating factor
       (MAF), a protein with N-acetylgalactosamine as the remaining sugar
       moiety. Thus, Gc protein is a precursor for MAF. Treatment of purified
       Gc protein with immobilized beta-galactosidase and sialidase generates
       an extremely high-titered MAF (GcMAF). When peripheral blood
       monocytes/macrophages of 46 HIV-infected patients were treated with
       GcMAF (100 pg/ml), the monocytes/macrophages of all patients were
       efficiently activated. However, the MAF precursor activity of plasma Gc
       protein was low in 16 (35%) of of these patients. Loss of the MAF
       precursor activity appeared to be due to deglycosylation of plasma Gc
       protein by alpha-N-acetylgalactosaminidase found in the patient blood
       stream. Levels of plasma alpha-N-acetylgalactosaminidase activity in
       individual patients had an inverse correlation with the MAF precursor
       activity of their plasma Gc protein. Thus, precursor activity of Gc
       protein and alpha-N-acetylgalactosaminidase activity in patient blood
       can serve as diagnostic and prognostic indices.
 DE    Acquired Immunodeficiency Syndrome/BLOOD/ENZYMOLOGY/*IMMUNOLOGY
       Galactosaminidase/*BLOOD  Glycosylation  Human  Immunosuppression
       Leukocytes, Mononuclear/IMMUNOLOGY  Lymphocytes/IMMUNOLOGY  Macrophage
       Activation  Macrophage-Activating Factors/*BLOOD  Macrophages/IMMUNOLOGY
       Substrate Specificity  Support, U.S. Gov't, P.H.S.  Vitamin D-Binding
       Protein/*BLOOD/CHEMISTRY  JOURNAL ARTICLE

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