Document 0718
 DOCN  M9650718
 TI    Helper and cytotoxic T cell responses of HIV type 1-infected individuals
       to synthetic peptides of HIV type 1 Rev.
 DT    9605
 AU    Blazevic V; Ranki A; Krohn KJ; Institute of Medical Technology,
       University of Tampere, Finland.
 SO    AIDS Res Hum Retroviruses. 1995 Nov;11(11):1335-42. Unique Identifier :
       AIDSLINE MED/96159130
 AB    In cell-mediated immunity T cells recognize peptide fragments of the
       antigenic protein in association with major histocompatibility complex
       (MHC) proteins. Synthetic 9- to 16-mer peptides have been widely used to
       identify the region(s) of a protein that act as T cell epitope. Here, we
       report antigenic peptides identified on HIV-1 regulatory protein Rev.
       Four synthetic peptides (amino acids 9-23, 25-39, 33-48, and 41-56) were
       first shown to stimulate T helper (Th) cell proliferation in peripheral
       blood lymphocytes (PBLs) derived from HIV-seropositive (HIV+)
       individuals. The same peptides induced cytotoxic T lymphocyte (CTL)
       activities toward the autologous target cells incubated with the
       peptides. Both responses were specific to the HIV infection as
       HIV-seronegative (HIV-) control individuals showed no significant
       proliferative or cytotoxic activity. The proliferating cells were CD4+ T
       cells, and CTL activity was mediated by CD8+ human leukocyte antigen
       (HLA)-restricted T cells. The identification of peptides containing
       epitopes that can induce both Th and CTL responses to regulatory
       proteins of HIV-1 in infected individuals might be important for vaccine
       development against AIDS. Since early regulatory proteins of HIV are
       expressed by the infected cells before the initiation of the synthesis
       of structural proteins, a CTL response against these proteins could
       destroy the infected cells before the release of infectious virions.
 DE    Amino Acid Sequence  Binding Sites  Gene Products, rev/CHEMICAL
       SYNTHESIS/*IMMUNOLOGY  Human  HIV Infections/*IMMUNOLOGY
       HIV-1/*IMMUNOLOGY  HLA Antigens/IMMUNOLOGY  Immunity, Cellular
       Immunodominant Epitopes/IMMUNOLOGY  Leukocytes, Mononuclear/IMMUNOLOGY
       Lymphocyte Transformation/IMMUNOLOGY  Molecular Sequence Data
       Peptides/CHEMICAL SYNTHESIS/IMMUNOLOGY  Support, Non-U.S. Gov't
       T-Lymphocytes, Cytotoxic/*IMMUNOLOGY  T-Lymphocytes,
       Helper-Inducer/*IMMUNOLOGY  JOURNAL ARTICLE

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