Document 0719
 DOCN  M9650719
 TI    Expansion of CD11abright cells in CD8+CD45RA+ from HIV-infected
       patients: a new early marker for disease progression?
 DT    9605
 AU    Scala E; Ansotegui IJ; Bellioni B; Guerra EC; Aiuti F; Paganelli R;
       Department of Clinical Medicine, University of Rome, La Sapienza,;
       Italy.
 SO    AIDS Res Hum Retroviruses. 1995 Nov;11(11):1327-33. Unique Identifier :
       AIDSLINE MED/96159129
 AB    CD8+CD45RA+ T lymphocytes present two distinct subpopulations expressing
       the CD11a molecule (LFA-1 alpha chain) with different intensity.
       CD11adim cells represent the unprimed population within the CD8+CD45RA+
       subset, whereas CD11abright cells are activated and may be considered as
       memory lymphocytes. The aim of this study was to analyze the expression
       of CD11a and CD18 within the CD8+CD45RA+ population in young
       HIV-infected individuals at different stages of disease as a marker of
       activation and of disease progression. Blood cells from 82 HIV-infected
       individuals and 23 age-matched healthy controls were stained with
       unconjugated CD11a, CD18, PE-goat F(ab')2 anti-mouse, FITC-CD45RA, and
       TRI-Color CD8. Quantitative analysis for three-color immunofluorescence
       was carried out by flow cytometry. HIV+ subjects were subdivided into
       three groups according to their CD4+ cell number (group A, CD4+ cells >
       500/microliters [20 subjects], group B, CD4+ cells between 500 and
       200/microliters [36 subjects], and group C, CD4+ lymphocytes <
       200/microliters [26 subjects]). We found a significant increase of
       CD11abright in the CD8+CD45RA+ subpopulation throughout the progression
       of the disease. The CD11abright percentage of positivity (mean) within
       the CD8+CD45RA+ subpopulation was 31% in healthy donors, 51% in group A,
       52% in group B, and 68% in group C. CD11abright expression was closely
       related to CD18bright (p < 0.001), but not to CD38. The relative
       increase of CD11a and CD18 expression in CD8+CD45RA+ T lymphocytes
       parallels the decrease of CD4+ cells and the progression of disease: an
       inverse correlation between the percentages of CD4+ cells/microliter and
       CD8+CD45RA+CD11abright cells (p < 0.001) and a direct correlation
       between the number of CD4+ lymphocytes per microliter and both the
       number of CD8+CD45RA+CD11adim cells (p < 0.001) and the number of
       CD8+CD45RA+CD11abright (p = 0.002) was observed. The relative increase
       of CD8+CD45RA+CD11abright cells may represent an additional marker for
       monitoring HIV-induced immunodeficiency.
 DE    Adult  Antigens, CD11/*METABOLISM  Antigens, CD18/*METABOLISM  Antigens,
       CD45/*IMMUNOLOGY  Biological Markers  CD4-CD8 Ratio  CD8-Positive
       T-Lymphocytes/*IMMUNOLOGY  Disease Progression  Female  Human  HIV
       Infections/BLOOD/*IMMUNOLOGY  Lymphocyte Transformation  Male  Middle
       Age  Support, Non-U.S. Gov't  JOURNAL ARTICLE

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