Document 0720
 DOCN  M9650720
 TI    Suppression of activation of the human immunodeficiency virus long
       terminal repeat by CD8+ T cells is not lentivirus specific.
 DT    9605
 AU    Copeland KF; McKay PJ; Rosenthal KL; Department of Pathology, McMaster
       University Health Sciences; Centre, Hamilton, Ontario, Canada.
 SO    AIDS Res Hum Retroviruses. 1995 Nov;11(11):1321-6. Unique Identifier :
       AIDSLINE MED/96159128
 AB    CD8+ T lymphocytes of HIV-1-infected individuals can efficiently
       suppress HIV-1 replication in CD4+ T lymphocytes. To elucidate the
       molecular events underlying this suppression, we have used the HIV-1 LTR
       directing the chloramphenicol acetyltransferase gene (CAT) in transient
       transfection assays using human Jurkat T cells. In addition to
       supernatants of patient CD8+ T lymphocytes (CD4+ > 350/microliters),
       supernatant of a T cell clone derived by Herpesvirus saimiri
       (HVS)-mediated transformation of CD8+ T lymphocytes of a patient
       demonstrating inhibition of virus replication were examined. Similar
       levels of inhibition of LTR-mediated gene expression in response to Tat
       or mitogenic activation with phorbol ester and calcium ionophore were
       observed by supernatants of both sources. The inhibitory effect of CD8+
       T lymphocytes was not exclusive to lentiviral LTRs since transcription
       of both the HTLV-I LTR and RSV LTR in response to mitogen was
       effectively inhibited. In examination of the influence of CD8+ T
       cell-derived supernatant on NF kappa B-mediated activation, a dimer of
       the HIV-1 NF kappa B elements directing CAT was markedly inhibited by
       supernatants of both patient CD8+ lymphocytes and the HVS-derived CD8+
       clone. Thus the inhibitory nature of CD8+ T lymphocytes appears not to
       be specific to lentiviral promoters and may mediate an inhibitory effect
       via the NF kappa B element.
 DE    Cell Line  CD8-Positive T-Lymphocytes/*IMMUNOLOGY  Gene Expression
       Regulation, Viral/IMMUNOLOGY  Herpesvirus 2, Saimirine/GENETICS  Human
       HIV/GENETICS/IMMUNOLOGY  HIV Long Terminal Repeat/*GENETICS/IMMUNOLOGY
       Lentivirus/GENETICS  Support, Non-U.S. Gov't  Transcription, Genetic
       JOURNAL ARTICLE

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