Document 0727
 DOCN  M9650727
 TI    Human herpesvirus 6 variant A, but not variant B, infects EBV-positive B
       lymphoid cells, activating the latent EBV genome through a
       BZLF-1-dependent mechanism.
 DT    9605
 AU    Cuomo L; Angeloni A; Zompetta C; Cirone M; Calogero A; Frati L; Ragona
       G; Faggioni A; Dipartimento di Medicina Sperimentale, Universita La
       Sapienza,; Rome, Italy.
 SO    AIDS Res Hum Retroviruses. 1995 Oct;11(10):1241-5. Unique Identifier :
       AIDSLINE MED/96157213
 AB    Human herpesvirus 6, a predominantly T lymphotropic virus, has been
       recently shown to infect some EBV-positive B cell lines, and to induce
       in them the activation of the EBV lytic cycle. Here we have confirmed
       and extended such observations, showing that (1) this phenomenon is
       restricted to the variant A of HHV-6: in fact two isolates belonging to
       the HHV-6 variant B (BA92 and Z29) were neither able to infect any B
       cell line, independently of the EBV status, nor to induce the EBV genome
       expression. The only exception is represented by the P3HR1 cells, in
       which, however, the infection by the variant B does not determine
       induction of EBV antigens; (2) the presence of the EBV genome
       contributes to the susceptibility of the B cell lines to HHV-6
       infection, increasing the binding sites and the percentage of infectable
       cells, as detected by immunoelectron microscopy; and (3) HHV-6 infected
       T cells, transfected with plasmids bearing the promoter regions of the
       EBV early genes BZLF1 and BMRF1, show a strong transactivation of these
       promoters.
 DE    B-Lymphocytes/*VIROLOGY  Cell Line  DNA-Binding Proteins/METABOLISM
       Heating  Herpesvirus 4, Human/GENETICS/*PHYSIOLOGY  Herpesvirus 6,
       Human/GENETICS/*PHYSIOLOGY/RADIATION EFFECTS  Human  Support, Non-U.S.
       Gov't  Trans-Activators/METABOLISM  Ultraviolet Rays  Variation
       (Genetics)  *Virus Activation  *Virus Latency  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).