Document 0728
 DOCN  M9650728
 TI    Investigation of proviral load in individuals infected with human
       T-lymphotropic virus type II.
 DT    9605
 AU    Woods TC; Graber JM; Hershow RC; Khabbaz RF; Kaplan JE; Heneine W;
       Retrovirus Diseases Branch, Centers for Disease Control and; Prevention,
       Atlanta, Georgia 30333, USA.
 SO    AIDS Res Hum Retroviruses. 1995 Oct;11(10):1235-9. Unique Identifier :
       AIDSLINE MED/96157212
 AB    Human T-lymphotrophic virus type II (HTLV-II) has not yet been
       associated with any disease. Little is known about the proviral loads of
       HTLV-II in vivo and its relationship, if any, to lack of pathogenicity.
       We determined the HTLV-II proviral copy number in peripheral blood
       lymphocyte (PBL) samples from 49 HTLV-II-infected individuals, of whom
       25 were coinfected with human immunodeficiency virus type 1 (HIV-1). The
       HTLV-II copy numbers were determined by polymerase chain reaction (PCR)
       amplification of end-point dilutions of PBL lysates, followed by
       hybridization to a 32P-labeled HTLV-II-specific probe. The proviral copy
       number for the 49 samples ranged from < 0.02 to 200 per 1000 PBLs; 6%
       had < 0.02, 16% had 0.02, 20% had 0.2, 18% had 2, 31% had 20, and 8% had
       200 copies per 1000 PBLs. The distributions of HTLV-II copy numbers in
       the coinfected and singly infected subgroups were not significantly
       different (Wilcoxon rank sum, p = 0.24). In the coinfected subgroup,
       there was no significant correlation between the HTLV-II proviral load
       and the counts of CD4-positive lymphocytes or CD8-positive lymphocytes
       (Spearman Coefficient = 0.26, p = 0.20; = 0.091, p = 0.67,
       respectively). Our data demonstrate the presence of a wide range of
       viral loads in HTLV-II-infected individuals. The high viral loads (> or
       = 20 copies/1000 lymphocytes) detected in 39% of our samples suggest
       that the low pathogenicity of HTLV-II is not related to the presence of
       low viral loads in the infected subjects. Our data from the HIV-1
       coinfected individuals show no apparent effect of HIV-1 on HTLV-II
       proviral loads.
 DE    Adult  CD4 Lymphocyte Count  CD8-Positive T-Lymphocytes  Human  HIV
       Infections/BLOOD/COMPLICATIONS  HIV-1  HTLV-II/*ISOLATION & PURIF
       HTLV-II Infections/BLOOD/COMPLICATIONS/*VIROLOGY  Lymphocyte Count
       Lymphocytes/VIROLOGY  Middle Age  Proviruses/*ISOLATION & PURIF  JOURNAL
       ARTICLE

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