Document 0732
 DOCN  M9650732
 TI    Plasma viremia titration and RNA quantitation in ICD-p24 negative HIV
       type-1-infected patients.
 DT    9605
 AU    Ercoli L; Sarmati L; el-Sawaf G; Cochi S; Lanti T; Iudicone P;
       Guglielmetti M; Giannini G; Galluzzo C; Tomino C; et al; Department of
       Public Health and Cellular Biology, University Tor; Vergata, Rome,
       Italy.
 SO    AIDS Res Hum Retroviruses. 1995 Oct;11(10):1203-7. Unique Identifier :
       AIDSLINE MED/96157208
 AB    Quantitative culture of human immunodeficiency virus (HIV) was performed
       on 202 plasma samples obtained from asymptomatic and early symptomatic
       HIV-1 infected patients (mean CD4+ count: 186/mm3) before antiretroviral
       therapy was started. HIV could be isolated from 84% of the plasma
       samples (titers ranging from 10(0) to 10(2.75) TCID50/ml). Immune
       complex dissociated p24 antigen (ICD-p24) was detected in 66% of the
       samples. Only 23 samples (11%) were negative for both ICD-p24 as well as
       HIV culture. Discordant results were obtained in 55 samples, and 45
       samples negative for ICD-p24 were positive for HIV culture. A
       significant proportion (42%) of patients that were negative for ICD-p24
       belonged to a very advanced group with very low CD4+ cell count.
       However, almost 90% of these ICD-p24 negative samples were positive for
       HIV plasma viremia, stressing the value of this virological marker in
       patients with low CD4+ cell count and without any detectable ICD-p24
       antigenemia. HIV-1 RNA was detected in all ICD-p24 negative plasma
       samples tested by the branched DNA (bDNA) assay. A very good correlation
       was found between high RNA copy number and HIV plasma isolation in
       samples obtained from patients with low CD4+ cell count, suggesting that
       HIV-1 RNA quantitation may also reflect viral infectivity of plasma.
 DE    Antigen-Antibody Complex/BLOOD  Antigens, Viral/BLOOD  CD4 Lymphocyte
       Count  Human  HIV Core Protein p24/*BLOOD  HIV-1/GENETICS/*ISOLATION &
       PURIF  RNA, Viral/*BLOOD  Support, Non-U.S. Gov't  Viremia/*VIROLOGY
       JOURNAL ARTICLE

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