Document 0782
 DOCN  M9650782
 TI    Inhibition of HIV-1 in vitro by C-5 propyne phosphorothioate antisense
       to rev.
 DT    9605
 AU    Lund OS; Nielsen JO; Hansen JE; Laboratory for Infectious Diseases,
       Department 144, Hvidovre; Hospital, Denmark.
 SO    Antiviral Res. 1995 Sep;28(1):81-91. Unique Identifier : AIDSLINE
       MED/96105499
 AB    A 15-mer C-5 propyne modified phosphorothioate oligodeoxynucleotide
       antisense to rev was approximately 5-fold more effective in providing
       viral inhibition compared to a 28-mer unmodified phosphorothioate
       oligodeoxynucleotide targeted to the same sequence and previously shown
       to inhibit HIV-1 in a sequence-dependent manner. The antiviral effect
       was obtained by lipofection or simple addition of 0.2-1 microM modified
       oligodeoxynucleotide to the culture medium of H9 cells chronically
       infected with the HIV-1LAI isolate of human immunodeficiency virus type
       1. We conclude that C-5 propyne oligodeoxynucleotides in accordance with
       previous findings by others are superior to unmodified phosphorothioates
       in providing inhibition of HIV-1 in a sequence-dependent manner and that
       this inhibition can be conferred by oligodeoxynucleotides in free
       solution.
 DE    Antiviral Agents/*PHARMACOLOGY  Base Sequence  *Genes, rev  Human  HIV
       Antigens/ANALYSIS  HIV-1/*DRUG EFFECTS  Molecular Sequence Data
       Oligonucleotides, Antisense/CHEMISTRY/*PHARMACOLOGY
       Phosphatidylethanolamines/PHARMACOLOGY  RNA, Viral  Structure-Activity
       Relationship  Support, Non-U.S. Gov't
       Thionucleotides/CHEMISTRY/*PHARMACOLOGY  Time Factors  Tumor Cells,
       Cultured  JOURNAL ARTICLE

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