Document 0865
 DOCN  M9650865
 TI    Lymphocyte traffic through lymph nodes and Peyer's patches of the rat:
       B- and T-cell-specific migration patterns within the tissue, and their
       dependence on splenic tissue.
 DT    9605
 AU    Blaschke V; Micheel B; Pabst R; Westermann J; Zentrum Anatomie 4120,
       Medizinische Hochschule Hannover, D-30623; Hannover, Germany.
 SO    Cell Tissue Res. 1995 Dec;282(3):377-86. Unique Identifier : AIDSLINE
       MED/96065187
 AB    The migration routes of lymphocyte subsets through organ compartments
       are of importance when trying to understand the local events taking
       place during immune responses. We have therefore studied the traffic of
       B, T, CD4(+), and CD8(+ )lymphocytes through lymph nodes and Peyer s
       patches. At various time points after injection into the rat, labeled
       lymphocytes were localized, and their phenotype characterized in
       cryostat sections using immunohistochemistry. Morphometry was also
       performed, and the recovery of 51Cr-labeled lymphocytes in these organs
       was determined. B and T lymphocytes entered the lymph nodes via the high
       endothelial venules in similar numbers. Most B lymphocytes migrated via
       the paracortex (T cell area) into the cortex (B cell area), and then
       back in substantial numbers into the paracortex. In contrast, T
       lymphocytes predominantly migrated into the paracortex and were rarely
       seen in the cortex. No obvious differences were seen between various
       lymph nodes and Peyer s patches and the routes of CD4(+) and
       CD8(+)lymphocytes. After injection of lymphocytes into animals with
       autotransplanted splenic tissue, the number of B lymphocytes that had
       migrated into the B cell area of lymph nodes and of Peyer s patches was
       significantly decreased, whereas CD4(+) lymphocytes migrated in larger
       numbers into the T cell area of both organs.
 DE    Animal  B-Lymphocytes/*CYTOLOGY  Cell Adhesion Molecules/METABOLISM
       Cell Count  Cell Movement  CD4-Positive T-Lymphocytes/CYTOLOGY
       CD8-Positive T-Lymphocytes/CYTOLOGY  Lymph Nodes/*CYTOLOGY  Lymphocyte
       Count  Male  Peyer's Patches/*CYTOLOGY  Rats  Rats, Inbred Lew
       Spleen/*CYTOLOGY/SURGERY  Support, Non-U.S. Gov't
       T-Lymphocytes/*CYTOLOGY  Transplantation, Autologous  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
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