Document 0866
 DOCN  M9650866
 TI    Selective restimulation of antigen or allergen preactivated T cells
       using OKT3 F(ab)2 results in the secretion of TH-1 or TH-2-like cytokine
       patterns.
 DT    9605
 AU    Jutel M; Wyss-Coray T; Carballido JM; Blaser K; Muller UR; Pichler WJ;
       Zieglerspital, Bern, Switzerland.
 SO    Clin Exp Allergy. 1995 Nov;25(11):1108-17. Unique Identifier : AIDSLINE
       MED/96161002
 AB    BACKGROUND: The synthesis of IgE is regulated by cytokines secreted from
       T-helper cells. The studies on cytokine secretion by peripheral blood
       mononuclear cells (PBMC) upon stimulation with antigen or allergen are
       difficult due to low levels of cytokines, especially of interleukin-4
       (IL-4). OBJECTIVE: In this study we tried to establish a culture system,
       which could enable the measurement of the cytokine profiles in
       specifically activated cultures. METHODS: Three methods to potentiate
       cytokine secretion were evaluated: PBMC from bee venom or house dust
       mite (Dermatophagoides pteronyssinus) allergic patients as well as
       normal subjects were stimulated either with the major bee venom allergen
       phospholipase A2 (PLA) or with the major D. pteronyssinus allergen (Der
       p 1) or with the control antigens tetanus toxoid (TT) and purified
       protein derivate (PPD). After 7 days of culture the cells were
       restimulated either with plastic bound OKT3 F(ab)2 monoclonal antibodies
       (MoAbs), with the appropriate antigen + antigen presenting cells or with
       IL-2. The secretion of cytokines (IL-4, IFN gamma) was measured after
       restimulation of the cultures (day 8). RESULTS: While OKT3 F(ab)2 was
       unable to activate resting T cells, it could restimulate preactivated
       cells. Restimulation with OKT3 F(ab)2 induced higher IL-4 and IFN gamma
       secretion than restimulation with IL-2 or antigen. TT and PLA stimulated
       a similar cytokine secretion profile in normal and PLA allergic subjects
       with substantial levels of both IL-4 and IFN gamma. In contrast, PPD
       induced virtually only IFN gamma secretion. Der p 1 stimulated mainly
       IL-4 secretion but also IFN gamma production in some mite allergic
       patients. CONCLUSION: We have established a cell culture system, which
       combines antigen specificity with a strong cytokine inducing signal
       provided by anti-CD3 MoAbs. TH-1 and TH-2 characteristic cytokine
       patterns can be observed in short-term PBMC cultures already after 8
       days of culture.
 DE    Allergens/*PHARMACOLOGY  Cells, Cultured  Cytokines/*SECRETION
       Epitopes/PHARMACOLOGY  Human  *Immunization  Immunoglobulins,
       Fab/*PHARMACOLOGY  Interleukin-2/IMMUNOLOGY/PHARMACOLOGY  Leukocytes,
       Mononuclear/IMMUNOLOGY  Lymphocyte Transformation
       Muromonab-CD3/CHEMISTRY/*PHARMACOLOGY  Phospholipases A/PHARMACOLOGY
       Support, Non-U.S. Gov't  Tetanus Toxoid/PHARMACOLOGY  Th1
       Cells/*SECRETION  Th2 Cells/*SECRETION  Tuberculin/PHARMACOLOGY  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).