Document 0877
 DOCN  M9650877
 TI    Isolation of anti-HIV-1 lignans from Larrea tridentata by
       counter-current chromatography.
 DT    9605
 AU    Gnabre JN; Ito Y; Ma Y; Huang RC; Department of Biology, Johns Hopkins
       University, Baltimore, MD; 21218-2685, USA.
 SO    J Chromatogr A. 1996 Jan 8;719(2):353-64. Unique Identifier : AIDSLINE
       MED/96155178
 AB    Several lignans, mostly new, were isolated from Larrea tridentata by
       assay-guided counter-current chromatography (CCC). Using the secreted
       alkaline phosphatase bioassay of HIV Tat transactivation and the
       two-phase hexane-ethyl acetate-methanol-water solvent system, two major
       components (Gr and Lo) were identified as anti-HIV active principles.
       The chemical structures of the constituents of Gr (G1-G4) and Lo (L1-L4)
       were determined by GC-MS and NMR. After optimization of isolation
       conditions, a large-scale isolation with the chloroform-methanol-water
       system yielded five constituents (FB1-FB5). The most predominant
       anti-HIV compound FB2 (denoted Malachi 4:5-6 or mal.4), which occurs in
       0.23% yield, was separated from its FB1 isomer (0.13% yield). Compound
       FB4 and two tricyclic lignans (FB3 and FB5) were also isolated in a
       substantial amount for further testing of their anti-HIV activities.
       These compounds may represent a new class of anti-HIV agents with
       important clinical relevance.
 DE    Antiviral Agents/*ISOLATION & PURIF/PHARMACOLOGY  Chromatography, Thin
       Layer/*METHODS  Gene Products, tat/PHYSIOLOGY  HIV-1/*DRUG EFFECTS
       Lignans/*ISOLATION & PURIF/PHARMACOLOGY  Mass Fragmentography  Nuclear
       Magnetic Resonance  Pilot Projects  Plants, Medicinal/*CHEMISTRY
       Support, Non-U.S. Gov't  Support, U.S. Gov't, Non-P.H.S.  Support, U.S.
       Gov't, P.H.S.  Trans-Activation (Genetics)/PHYSIOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).