Document 0895
 DOCN  M9650895
 TI    Proinflammatory cytokine expression in cervicovaginal secretions of
       normal and HIV-infected women.
 DT    9605
 AU    Belec L; Gherardi R; Payan C; Prazuck T; Malkin JE; Tevi-Benissan C;
       Pillot J; Unite d'Immunologie Microbienne, Institut Pasteur, Paris,;
       France.
 SO    Cytokine. 1995 Aug;7(6):568-74. Unique Identifier : AIDSLINE
       MED/96100024
 AB    Proinflammatory cytokines may stimulate replication and spread of HIV.
       To evaluate to what extent the female genital tract represents a source
       of these cytokines, we determined TNF-alpha, IL-1 beta and IL-6
       concentrations in paired serum and cervicovaginal washings from 45
       HIV-negative and 50 HIV-positive women, and then we looked for the
       relevant mRNAs in cervicovaginal secretions by RT-PCR. Cytokines were
       detected by ELISA in cervicovaginal fluid from most healthy women.
       Cervicovaginal washing levels of TNF-alpha were increased above the
       control value +2 SD in 11/50 HIV-positive women, those of IL-1 beta in
       13/50, and those of IL-6 in 14/50. The prevalences of TNF-alpha, IL-1
       beta and IL-6 increase and their levels in cervicovaginal washings were
       significantly higher in the 20 patients at stage IV than in the 30
       patients at earlier stages of the disease. In HIV-infected patients,
       serum and cervicovaginal washing levels correlated positively for
       TNF-alpha and IL-6, but not for IL-1 beta. Nine of 15 cytokine mRNAs
       determinations were positive in HIV-infected women versus 1 of 15 in
       controls (P < 0.01). These findings could be relevant to bidirectional
       heterosexual transmission of HIV.
 DE    Case-Control Studies  Cervix Uteri/*SECRETION
       Cytokines/BLOOD/*SECRETION  Female  Human  HIV
       Infections/BLOOD/*PHYSIOPATHOLOGY/TRANSMISSION
       Inflammation/*PHYSIOPATHOLOGY  Interleukin-1/BLOOD/SECRETION
       Interleukin-6/BLOOD/SECRETION  Reference Values  RNA,
       Messenger/BIOSYNTHESIS  Support, Non-U.S. Gov't  Tumor Necrosis
       Factor/SECRETION  Vagina/*SECRETION  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).