Document 0897
 DOCN  M9650897
 TI    Genome analysis of North American small ruminant lentiviruses by
       polymerase chain reaction and restriction enzyme analysis.
 DT    9605
 AU    Rosati S; Kwang J; Keen JE; Department of Animal Production,
       Epidemiology and Ecology,; University of Turin, Italy.
 SO    J Vet Diagn Invest. 1995 Oct;7(4):437-43. Unique Identifier : AIDSLINE
       MED/96155806
 AB    The polymerase chain reaction (PCR) was used to amplify portions of the
       gag and env structural genes of 8 ovine and 1 caprine lentivirus
       isolates of North American origin. Three sets of primers were used to
       amplify p16, p25, and N'-gp40 gene fragments, and 1 set, annealing
       highly conserved portions of long terminal repeat (LTR) among small
       ruminant lentiviruses, was used as a positive control. Variable PCR
       amplification efficiency was observed. Different stringency conditions
       of hybridization with specific DNA probes were used to maximize
       detection of the PCR product. The p25 primers detected all strains, the
       gp40 primers detected 1 ovine and the caprine strain, and the p16
       primers detected only 1 ovine isolate. All strains were detected by LTR
       primers. Restriction endonuclease analysis of 5 amplified p25 and 2
       N'-gp40 gene fragments revealed extensive heterogeneity among these
       North American small ruminant lentiviruses.
 DE    Animal  Base Sequence  Comparative Study  DNA Primers  DNA,
       Viral/ISOLATION & PURIF  Genes, env  Genes, gag  Goats/*VIROLOGY
       Lentiviruses, Ovine-Caprine/GENETICS/*ISOLATION & PURIF  Molecular
       Sequence Data  North America  Polymerase Chain Reaction/*METHODS
       Repetitive Sequences, Nucleic Acid  *Restriction Mapping
       Ruminants/VIROLOGY  Sensitivity and Specificity  Sheep/*VIROLOGY
       Species Specificity  Support, Non-U.S. Gov't  JOURNAL ARTICLE

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