Document 0899
 DOCN  M9650899
 TI    In vitro antiviral activities of sulfated polysaccharides from a marine
       microalga (Cochlodinium polykrikoides) against human immunodeficiency
       virus and other enveloped viruses.
 DT    9605
 AU    Hasui M; Matsuda M; Okutani K; Shigeta S; Faculty of Agriculture, Kagawa
       University, Japan.
 SO    Int J Biol Macromol. 1995 Oct;17(5):293-7. Unique Identifier : AIDSLINE
       MED/96121688
 AB    A marine microalga, Cochlodinium polykrikoides, produces extracellular
       sulfated polysaccharides. Isolation and purification of the
       polysaccharides were accomplished by precipitation with ethanol and
       Cetavlon, followed by DEAE-cellulose column chromatography
       (polysaccharides A1 and A2). These polysaccharides, which were
       homogeneous when analysed by both ultracentrifugal and electrophoretic
       methods, were composed of mannose, galactose, glucose and uronic acid,
       together with sulfate groups (S = 7-8% w/w). Both A1 and A2 inhibited
       the cytopathic effect of influenza virus types A and B in MDCK cells,
       that of respiratory syncytial virus types A and B in HEp-2 cells, that
       of human immunodeficiency virus type 1 in MT-4 cells; and, except A1 for
       herpes simplex virus type 1 and A2 for parainfluenza virus type 2 in
       HMV-2 cells, the cochlodinium polysaccharides showed no antiviral
       activity against parainfluenza virus types 2 and 3, measles virus, mumps
       virus or herpes simplex virus type 1 in HMV-2 cells. No cytotoxicity for
       host cells was observed with these polysaccharides at a concentration of
       100 micrograms ml-1. Inhibitory effects on various viruses were achieved
       at concentrations that were not markedly inhibitory to the blood
       coagulation process.
 DE    Algae/*CHEMISTRY  Animal  Antiviral Agents/ISOLATION &
       PURIF/*PHARMACOLOGY  Cell Division/DRUG EFFECTS  Cell Line/DRUG
       EFFECTS/VIROLOGY  Chromatography/METHODS  Drug Screening  Human
       HIV-1/*DRUG EFFECTS  Kidney/CYTOLOGY/VIROLOGY  Laryngeal
       Neoplasms/PATHOLOGY  Orthomyxoviruses Type A/DRUG EFFECTS
       Polysaccharides/CHEMISTRY/*ISOLATION & PURIF/*PHARMACOLOGY  Respiratory
       Syncytial Viruses/DRUG EFFECTS  Simplexvirus/DRUG EFFECTS
       Spectrophotometry, Infrared  Sulfates  Support, Non-U.S. Gov't  Tumor
       Cells, Cultured  Virus Replication/DRUG EFFECTS  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).