Document 0901
 DOCN  M9650901
 TI    The protein tyrosine kinase Fyn activates transcription from the HIV
       promoter via activation of NF kappa B-like DNA-binding proteins.
 DT    9605
 AU    Hohashi N; Hayashi T; Fusaki N; Takeuchi M; Higurashi M; Okamoto T;
       Semba K; Yamamoto T; Department of Oncology, University of Tokyo, Japan.
 SO    Int Immunol. 1995 Nov;7(11):1851-9. Unique Identifier : AIDSLINE
       MED/96162443
 AB    Protein tyrosine kinase p59fyn (Fyn) associates with the TCR-CD3
       complex, which suggests that Fyn plays a significant role in the signal
       transduction involving TCR complex. In addition to cellular genes, viral
       promoters such as the HIV long terminal repeat (LTR) are also activated
       upon T cell activation. To elucidate the functional significance of Fyn
       in the expression of viral promoters, we transfected a Fyn-expression
       vector together with a reporter plasmid containing the chloramphenicol
       acetyltransferase gene driven by HIV LTR into a human T cell line,
       Jurkat. In this assay, Fyn stimulated the promoter in HIV LTR when the
       transfected cells were treated with both concanavalin A and PMA as an
       antigen-mimic stimulation. This activation required the intact SH2
       domain of Fyn. Mutational analysis of HIV LTR showed that the NF kappa B
       binding sites were responsible for this effect. Electrophoretic mobility
       shift assays and UV cross-linking experiments showed that activation of
       T cells by anti-CD3 antibody induced four kappa B-binding proteins (50,
       60, 65 and 100 kDa) in Fyn-overexpressing cells more efficiently than in
       the parental cells. Our results suggested that Fyn was able to regulate
       expression of a subset of genes via kappa B-binding proteins upon T cell
       activation.
 DE    Base Sequence  Binding Sites/GENETICS  DNA-Binding
       Proteins/BIOSYNTHESIS/*GENETICS  *Gene Expression Regulation  Human
       HIV/*GENETICS  Leukemia, T-Cell  Molecular Sequence Data  NF-kappa
       B/BIOSYNTHESIS/*GENETICS  Promoter Regions (Genetics)/*DRUG EFFECTS
       Protein-Tyrosine Kinase/*GENETICS/PHYSIOLOGY  Proto-Oncogene
       Proteins/*GENETICS/PHYSIOLOGY  Repetitive Sequences, Nucleic Acid/DRUG
       EFFECTS  Support, Non-U.S. Gov't  Trans-Activation (Genetics)/DRUG
       EFFECTS  Transcription, Genetic/*DRUG EFFECTS  Tumor Cells, Cultured
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
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