Document 0838
 DOCN  M9540838
 TI    Susceptibility of human T-lymphotropic virus type I infected cell line
       MT-2 to hepatitis C virus infection.
 DT    9504
 AU    Kato N; Nakazawa T; Mizutani T; Shimotohno K; Virology Division,
       National Cancer Center Research Institute,; Tokyo, Japan.
 SO    Biochem Biophys Res Commun. 1995 Jan 26;206(3):863-9. Unique Identifier
       : AIDSLINE GENBANK/D43656
 AB    To obtain a hepatitis C virus (HCV) proliferation system, we examined
       the susceptibility of various cultured cell lines to HCV infection. We
       found that a human T-lymphotropic virus type I infected cell line MT-2
       was fairly sensitive to HCV infection. Using the polymerase chain
       reaction, intracellular positive-stranded HCV RNA was detected until at
       least 15 days postinoculation (p.i.). Intracellular negative-stranded
       HCV RNA was also detected at 10 days p.i., although not at 7 days p.i.,
       suggesting that HCV is replicating in MT-2 cells 10 days p.i. Sequence
       analysis of hypervariable region 1 (HVR1) revealed that HVR1 sequences
       from cells 10 days p.i. had become homogeneous, although HVR1 sequences
       from the inoculum showed the typical quasi-species. We also found a lack
       of anti-HVR1 antibody against the HVR1 species which became homogeneous
       at 10 days p.i., although we easily detected antibody against the other
       HVR1 species obtained from the inoculum. These findings suggest that
       MT-2 cells are susceptible to HCV infection and are capable of
       supporting HCV replication.
 DE    Amino Acid Sequence  Animal  Base Sequence  Carcinoma, Hepatocellular
       Cell Line  Hepatitis C Viruses/*GROWTH & DEVELOPMENT/GENETICS  Human
       HTLV-I/*GROWTH & DEVELOPMENT  Kidney  Leukemia  Liver Neoplasms
       Molecular Sequence Data  Polymerase Chain Reaction  RNA, Viral/ANALYSIS
       Support, Non-U.S. Gov't  Swine  Tumor Cells, Cultured  Viral Envelope
       Proteins/ANALYSIS/CHEMISTRY/GENETICS  Virus Cultivation  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).